Primary hepatocytes: Current understanding of the regulation of metabolic enzymes and transporter proteins, and pharmaceutical practice for the use of hepatocytes in metabolism, enzyme induction, transporter, clearance, and hepatotoxicity studies

Nicola J. Hewitt, María José Gómez Lechón, J. Brian Houston, David Hallifax, Hayley S. Brown, Patrick Maurel, J. Gerald Kenna, Lena Gustavsson, Christina Lohmann, Christian Skonberg, Andre Guillouzo, Gregor Tuschl, Albert P. Li, Edward Lecluyse, Geny M M Groothuis, Jan G. Hengstler

    Research output: Contribution to journalArticlepeer-review

    Abstract

    This review brings you up-to-date with the hepatocyte research on: 1) in vitro-in vivo correlations of metabolism and clearance; 2) CYP enzyme induction, regulation, and cross-talk using human hepatocytes and hepatocyte-like cell lines; 3) the function and regulation of hepatic transporters and models used to elucidate their role in drug clearance; 4) mechanisms and examples of idiosyncratic and intrinsic hepatotoxicity; and 5) alternative cell systems to primary human hepatocytes. We also report pharmaceutical perspectives of these topics and compare methods and interpretations for the drug development process. Copyright © Informa Healthcare.
    Original languageEnglish
    Pages (from-to)159-234
    Number of pages75
    JournalDrug Metabolism Reviews
    Volume39
    Issue number1
    DOIs
    Publication statusPublished - Jan 2007

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