Probing bactericidal mechanisms induced by cold atmospheric plasmas with Escherichia coli mutants

Stefano Perni; Gilbert Shama; J. L. Hobman; P. A. Lund; C. J. Kershaw; G. A. Hidalgo-Arroyo; C. W. Penn; X. T. Deng; J. L. Walsh; M. G. Kong

doi:10.1063/1.2458162

Probing bactericidal mechanisms induced by cold atmospheric plasmas with Escherichia coli mutants

Stefano Perni, Gilbert Shama, J. L. Hobman, P. A. Lund, C. J. Kershaw, G. A. Hidalgo-Arroyo, C. W. Penn, X. T. Deng, J. L. Walsh, M. G. Kong

Research output: Contribution to journal › Article › peer-review

Abstract

Mechanisms of plasma-induced microbial inactivation have commonly been studied with physicochemical techniques. In this letter, Escherichia coli K-12 and its ΔrecA, ΔrpoS, and ΔsoxS mutants are employed to discriminate effects of UV photons, OH radicals, and reactive oxygen species produced in atmospheric discharges. This microbiological approach exploits the fact that these E. coli mutants are defective in their resistance against various external stresses. By interplaying bacterial inactivation kinetics with optical emission spectroscopy, oxygen atoms are identified as a major contributor in plasma inactivation with minor contributions from UV photons, OH radicals, singlet oxygen metastables, and nitric oxide. © 2007 American Institute of Physics.

Original language	English
Article number	073902
Journal	Applied Physics Letters
Volume	90
Issue number	7
DOIs	https://doi.org/10.1063/1.2458162
Publication status	Published - 2007

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@article{c70f91008c4240ea816571e83cb62f85,

title = "Probing bactericidal mechanisms induced by cold atmospheric plasmas with Escherichia coli mutants",

abstract = "Mechanisms of plasma-induced microbial inactivation have commonly been studied with physicochemical techniques. In this letter, Escherichia coli K-12 and its ΔrecA, ΔrpoS, and ΔsoxS mutants are employed to discriminate effects of UV photons, OH radicals, and reactive oxygen species produced in atmospheric discharges. This microbiological approach exploits the fact that these E. coli mutants are defective in their resistance against various external stresses. By interplaying bacterial inactivation kinetics with optical emission spectroscopy, oxygen atoms are identified as a major contributor in plasma inactivation with minor contributions from UV photons, OH radicals, singlet oxygen metastables, and nitric oxide. {\textcopyright} 2007 American Institute of Physics.",

author = "Stefano Perni and Gilbert Shama and Hobman, {J. L.} and Lund, {P. A.} and Kershaw, {C. J.} and Hidalgo-Arroyo, {G. A.} and Penn, {C. W.} and Deng, {X. T.} and Walsh, {J. L.} and Kong, {M. G.}",

note = "Times Cited: 49",

year = "2007",

doi = "10.1063/1.2458162",

language = "English",

volume = "90",

journal = "Applied Physics Letters",

issn = "0003-6951",

publisher = "American Institute of Physics",

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TY - JOUR

T1 - Probing bactericidal mechanisms induced by cold atmospheric plasmas with Escherichia coli mutants

AU - Perni, Stefano

AU - Shama, Gilbert

AU - Hobman, J. L.

AU - Lund, P. A.

AU - Kershaw, C. J.

AU - Hidalgo-Arroyo, G. A.

AU - Penn, C. W.

AU - Deng, X. T.

AU - Walsh, J. L.

AU - Kong, M. G.

N1 - Times Cited: 49

PY - 2007

Y1 - 2007

N2 - Mechanisms of plasma-induced microbial inactivation have commonly been studied with physicochemical techniques. In this letter, Escherichia coli K-12 and its ΔrecA, ΔrpoS, and ΔsoxS mutants are employed to discriminate effects of UV photons, OH radicals, and reactive oxygen species produced in atmospheric discharges. This microbiological approach exploits the fact that these E. coli mutants are defective in their resistance against various external stresses. By interplaying bacterial inactivation kinetics with optical emission spectroscopy, oxygen atoms are identified as a major contributor in plasma inactivation with minor contributions from UV photons, OH radicals, singlet oxygen metastables, and nitric oxide. © 2007 American Institute of Physics.

AB - Mechanisms of plasma-induced microbial inactivation have commonly been studied with physicochemical techniques. In this letter, Escherichia coli K-12 and its ΔrecA, ΔrpoS, and ΔsoxS mutants are employed to discriminate effects of UV photons, OH radicals, and reactive oxygen species produced in atmospheric discharges. This microbiological approach exploits the fact that these E. coli mutants are defective in their resistance against various external stresses. By interplaying bacterial inactivation kinetics with optical emission spectroscopy, oxygen atoms are identified as a major contributor in plasma inactivation with minor contributions from UV photons, OH radicals, singlet oxygen metastables, and nitric oxide. © 2007 American Institute of Physics.

U2 - 10.1063/1.2458162

DO - 10.1063/1.2458162

M3 - Article

SN - 0003-6951

VL - 90

JO - Applied Physics Letters

JF - Applied Physics Letters

IS - 7

M1 - 073902

ER -

Probing bactericidal mechanisms induced by cold atmospheric plasmas with Escherichia coli mutants

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