Probing eukaryotic genome functions with synthetic chromosomes

Zhouqing Luo; Stefan A Hoffmann; Shuangying Jiang; Yizhi Cai; Junbiao Dai

doi:10.1016/j.yexcr.2020.111936

Probing eukaryotic genome functions with synthetic chromosomes

Zhouqing Luo
, Stefan A Hoffmann
, Shuangying Jiang
, Yizhi Cai
, Junbiao Dai

Institute of Process Engineering, Chinese Academy of Sciences

Research output: Contribution to journal › Review article › peer-review

Abstract

The ability to redesign and reconstruct a cell at whole-genome level provides new platforms for biological study. The international synthetic yeast genome project-Sc2.0, designed by interrogating knowledge amassed by the yeast community to date, exemplifies how a classical synthetic biology "design-build-test-learn" engineering cycle can effectively test hypotheses about various genome fundamentals. The genome reshuffling SCRaMbLE system implemented in synthetic yeast strains also provides unprecedented diversified resources for genotype-phenotype study and yeast metabolic engineering. Further development of genome synthesis technology will shed new lights on complex biological processes in higher eukaryotes.

Original language	English
Pages (from-to)	111936
Journal	Experimental Cell Research
Volume	390
Issue number	1
Early online date	9 Mar 2020
DOIs	https://doi.org/10.1016/j.yexcr.2020.111936
Publication status	Published - 1 May 2020

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10.1016/j.yexcr.2020.111936

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title = "Probing eukaryotic genome functions with synthetic chromosomes",

abstract = "The ability to redesign and reconstruct a cell at whole-genome level provides new platforms for biological study. The international synthetic yeast genome project-Sc2.0, designed by interrogating knowledge amassed by the yeast community to date, exemplifies how a classical synthetic biology {"}design-build-test-learn{"} engineering cycle can effectively test hypotheses about various genome fundamentals. The genome reshuffling SCRaMbLE system implemented in synthetic yeast strains also provides unprecedented diversified resources for genotype-phenotype study and yeast metabolic engineering. Further development of genome synthesis technology will shed new lights on complex biological processes in higher eukaryotes.",

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N2 - The ability to redesign and reconstruct a cell at whole-genome level provides new platforms for biological study. The international synthetic yeast genome project-Sc2.0, designed by interrogating knowledge amassed by the yeast community to date, exemplifies how a classical synthetic biology "design-build-test-learn" engineering cycle can effectively test hypotheses about various genome fundamentals. The genome reshuffling SCRaMbLE system implemented in synthetic yeast strains also provides unprecedented diversified resources for genotype-phenotype study and yeast metabolic engineering. Further development of genome synthesis technology will shed new lights on complex biological processes in higher eukaryotes.

AB - The ability to redesign and reconstruct a cell at whole-genome level provides new platforms for biological study. The international synthetic yeast genome project-Sc2.0, designed by interrogating knowledge amassed by the yeast community to date, exemplifies how a classical synthetic biology "design-build-test-learn" engineering cycle can effectively test hypotheses about various genome fundamentals. The genome reshuffling SCRaMbLE system implemented in synthetic yeast strains also provides unprecedented diversified resources for genotype-phenotype study and yeast metabolic engineering. Further development of genome synthesis technology will shed new lights on complex biological processes in higher eukaryotes.

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Probing eukaryotic genome functions with synthetic chromosomes

Abstract

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