Production of a highly specific monoclonal antibody to progesterone

A. White, D. C. Anderson, J. R. Daly

    Research output: Contribution to journalArticlepeer-review


    A progesterone monoclonal antibody which may be used in the development of a sensitive and specific immunoassay is described. The preparation involved isolation of spleen cells from Balb/c mice immunized with progesterone 11α-hemisuccinate-bovine serum albumin conjugate, which were then fused with two different mouse myeloma cell lines. Hybridomas secreting antibody to progesterone were detected by immunoassay and cloned in soft agar. Five percent of the resulting clones secreted antibody; these clones originated from cultures producing very little antibody, and so might easily have been discarded. The monoclonal antibody obtained is highly specific and has a binding affinity of approximately 1.7 x 109 M-1, so it may be advantageously used in the detection of progesterone in body fluids.
    Original languageEnglish
    Pages (from-to)205-207
    Number of pages2
    JournalJournal of Clinical Endocrinology and Metabolism
    Issue number1
    Publication statusPublished - 1982


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