Production of essential oil constituents by cultured cells of Carum copticum L

Callus cultures of Carum copticum were established from cotyledons and healthy suspensions grown using Murashige and Skoog medium. The dichloromethane extract of cell suspension culture of Carum copticum was analysed by GC and GC-MS; 41 components were identified, of which the major constituents were found to be elemol (11.5%), α-cadinol (10.6%), δ-cadinene (7.8%), caryophyllene (6.2%), muurolol (4.9%), β-eudesmol (3.1%), β-elemene (3.9%), α-muurolene (2.6%), limonene (2.4%) and α-humulene (2.0%). Copyright © 2002 John Wiley & Sons, Ltd.