TY - JOUR
T1 - Production of Polyhydroxyalkanoates from Hydrolysed Rapeseed Meal by Haloferax mediterranei
AU - Khamplod, Thammarit
AU - Wongsirichot, Phavit
AU - Winterburn, James
PY - 2023/10/1
Y1 - 2023/10/1
N2 - Rapeseed meal (RSM) hydrolysate is a potential low-cost feedstock for the production of polyhydroxyalkanoates (PHAs) by the archaea, Haloferax mediterranei. Acidic and enzymatic hydrolysis were carried out to compare effectiveness. Enzymatic hydrolysis is more effective than acidic hydrolysis for fermentation substrate leading to increased PHA productivity. H. mediterranei didn’t grow or produce PHA when acid hydrolysed RSM medium was present in proportions greater than 25% (vol.), potentially due to the effect of inhibitors such as furfural, hydroxymethylfurfural (HMF), etc. However, H. mediterranei was able to grow and produce PHA when using enzymatically hydrolysed RSM medium. The maximum PHA concentration of 0.512 g/L was found at 75% (vol.) in enzymatic RSM hydrolysate medium. The biopolymer obtained had improved thermal and mechanical properties compared to PHB homopolymer. RSM’s potential as a low-cost alternative feedstock for improved PHA production under non-sterile conditions was successfully demonstrated, and its usage should be further explored.
AB - Rapeseed meal (RSM) hydrolysate is a potential low-cost feedstock for the production of polyhydroxyalkanoates (PHAs) by the archaea, Haloferax mediterranei. Acidic and enzymatic hydrolysis were carried out to compare effectiveness. Enzymatic hydrolysis is more effective than acidic hydrolysis for fermentation substrate leading to increased PHA productivity. H. mediterranei didn’t grow or produce PHA when acid hydrolysed RSM medium was present in proportions greater than 25% (vol.), potentially due to the effect of inhibitors such as furfural, hydroxymethylfurfural (HMF), etc. However, H. mediterranei was able to grow and produce PHA when using enzymatically hydrolysed RSM medium. The maximum PHA concentration of 0.512 g/L was found at 75% (vol.) in enzymatic RSM hydrolysate medium. The biopolymer obtained had improved thermal and mechanical properties compared to PHB homopolymer. RSM’s potential as a low-cost alternative feedstock for improved PHA production under non-sterile conditions was successfully demonstrated, and its usage should be further explored.
KW - Haloferax mediterranei
KW - Rapeseed meal
KW - Fermentation
KW - Polyhydroxyalkanoates
KW - Hydrolysis
U2 - 10.1016/j.biortech.2023.129541
DO - 10.1016/j.biortech.2023.129541
M3 - Article
SN - 0960-8524
JO - Bioresource Technology
JF - Bioresource Technology
ER -