Proline-conditioning and chemically-programmed ice nucleation protects spheroids during cryopreservation

Yanan Gao, Akalabya Bissoyi, Nina L. H. Kinney, Thomas F. Whale, Qiongyu Guo, Matthew I. Gibson

Research output: Contribution to journalArticlepeer-review

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Spheroids mimic 3-D tissue niches better than standard cell cultures. Cryopreserving spheroids, however, remains challenging as conventional cryoprotectants do not mitigate all damage mechanisms. Here chemically-programmed extracellular ice nucleation is used to prevent supercooling, alongside proline pre-conditioning, which are found to synergystically improve post-thaw recovery of spheroids. This validates the need to identify compounds and materials to address both biochemical and biophysical damage pathways beyond standard cryoprotectants.

Original languageEnglish
Pages (from-to)9086-9089
Number of pages4
JournalChemical Communications
Issue number59
Early online date4 Jul 2023
Publication statusPublished - 28 Jul 2023


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