Protecting monoclonal antibodies via competitive interfacial adsorption of nonionic surfactants

Zeyuan Zhuang; Haoran Wu; Zongyi Li; Mingrui Liao; Kangcheng Shen; Renzhi Li; Stephen Hall; Cavan Kalonia; Kai Tao; Xuzhi Hu; Jian Ren Lu

doi:10.1016/j.jcis.2024.12.214

Protecting monoclonal antibodies via competitive interfacial adsorption of nonionic surfactants

Zeyuan Zhuang, Haoran Wu, Zongyi Li, Mingrui Liao, Kangcheng Shen, Renzhi Li, Stephen Hall, Cavan Kalonia, Kai Tao, Xuzhi Hu, Jian Ren Lu

AstraZeneca

Research output: Contribution to journal › Article › peer-review

Abstract

Bioengineered monoclonal antibodies (mAbs) have gained significant recognition as medical therapies. However, during processing, storage and use, mAbs are susceptible to interfacial adsorption and desorption, leading to structural deformation and aggregation, and undermining their bioactivity. To suppress antibody surface adsorption, nonionic surfactants are commonly used in formulation. But how surface hydrophobicity affects the adsorption and desorption of mAbs and nonionic surfactants individually and as a mixture remains inconclusive.
Experiments
The rapid tuning of the siliconized surface from hydrophobic to hydrophilic was controlled by the UV oxidation time of a self-assembled trimethoxy(7-octen-1-yl)silane (TMOS) monolayer. Spectroscopic ellipsometry and neutron reflection were used to determine the dynamic adsorption and structural changes of the co-adsorbed mAb (COE-3) and …

Original language	English
Pages (from-to)	819-830
Number of pages	12
Journal	Journal of Colloid and Interface Science
Volume	684
Early online date	28 Dec 2024
DOIs	https://doi.org/10.1016/j.jcis.2024.12.214
Publication status	Published - 15 Apr 2025

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10.1016/j.jcis.2024.12.214Licence: CC BY

Structural changes of interfacially adsorbed antibody molecules
Lu, J. (PI) & Waigh, T. (CoI)
1/01/20 → 30/06/23
Project: Research

Cite this

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title = "Protecting monoclonal antibodies via competitive interfacial adsorption of nonionic surfactants",

abstract = "Bioengineered monoclonal antibodies (mAbs) have gained significant recognition as medical therapies. However, during processing, storage and use, mAbs are susceptible to interfacial adsorption and desorption, leading to structural deformation and aggregation, and undermining their bioactivity. To suppress antibody surface adsorption, nonionic surfactants are commonly used in formulation. But how surface hydrophobicity affects the adsorption and desorption of mAbs and nonionic surfactants individually and as a mixture remains inconclusive.ExperimentsThe rapid tuning of the siliconized surface from hydrophobic to hydrophilic was controlled by the UV oxidation time of a self-assembled trimethoxy(7-octen-1-yl)silane (TMOS) monolayer. Spectroscopic ellipsometry and neutron reflection were used to determine the dynamic adsorption and structural changes of the co-adsorbed mAb (COE-3) and …",

author = "Zeyuan Zhuang and Haoran Wu and Zongyi Li and Mingrui Liao and Kangcheng Shen and Renzhi Li and Stephen Hall and Cavan Kalonia and Kai Tao and Xuzhi Hu and Lu, {Jian Ren}",

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doi = "10.1016/j.jcis.2024.12.214",

language = "English",

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journal = "Journal of Colloid and Interface Science",

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TY - JOUR

T1 - Protecting monoclonal antibodies via competitive interfacial adsorption of nonionic surfactants

AU - Zhuang, Zeyuan

AU - Wu, Haoran

AU - Li, Zongyi

AU - Liao, Mingrui

AU - Shen, Kangcheng

AU - Li, Renzhi

AU - Hall, Stephen

AU - Kalonia, Cavan

AU - Tao, Kai

AU - Hu, Xuzhi

AU - Lu, Jian Ren

PY - 2025/4/15

Y1 - 2025/4/15

N2 - Bioengineered monoclonal antibodies (mAbs) have gained significant recognition as medical therapies. However, during processing, storage and use, mAbs are susceptible to interfacial adsorption and desorption, leading to structural deformation and aggregation, and undermining their bioactivity. To suppress antibody surface adsorption, nonionic surfactants are commonly used in formulation. But how surface hydrophobicity affects the adsorption and desorption of mAbs and nonionic surfactants individually and as a mixture remains inconclusive.ExperimentsThe rapid tuning of the siliconized surface from hydrophobic to hydrophilic was controlled by the UV oxidation time of a self-assembled trimethoxy(7-octen-1-yl)silane (TMOS) monolayer. Spectroscopic ellipsometry and neutron reflection were used to determine the dynamic adsorption and structural changes of the co-adsorbed mAb (COE-3) and …

AB - Bioengineered monoclonal antibodies (mAbs) have gained significant recognition as medical therapies. However, during processing, storage and use, mAbs are susceptible to interfacial adsorption and desorption, leading to structural deformation and aggregation, and undermining their bioactivity. To suppress antibody surface adsorption, nonionic surfactants are commonly used in formulation. But how surface hydrophobicity affects the adsorption and desorption of mAbs and nonionic surfactants individually and as a mixture remains inconclusive.ExperimentsThe rapid tuning of the siliconized surface from hydrophobic to hydrophilic was controlled by the UV oxidation time of a self-assembled trimethoxy(7-octen-1-yl)silane (TMOS) monolayer. Spectroscopic ellipsometry and neutron reflection were used to determine the dynamic adsorption and structural changes of the co-adsorbed mAb (COE-3) and …

U2 - 10.1016/j.jcis.2024.12.214

DO - 10.1016/j.jcis.2024.12.214

M3 - Article

SN - 0021-9797

VL - 684

SP - 819

EP - 830

JO - Journal of Colloid and Interface Science

JF - Journal of Colloid and Interface Science

ER -

Protecting monoclonal antibodies via competitive interfacial adsorption of nonionic surfactants

Abstract

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Structural changes of interfacially adsorbed antibody molecules

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