Protein-4.2 association with band 3 (AE1, SLCA4) in Xenopus oocytes: Effects of three natural protein-4.2 mutations associated with hemolytic anemia

Ashley M. Toye, Sandip Ghosh, Mark T. Young, Graham K. Jones, Richard B. Sessions, Martine Ramaugé, Philippe Leclerc, Joyoti Basu, Jean Delaunay, Michael J A Tanner

    Research output: Contribution to journalArticlepeer-review

    Abstract

    We have investigated the effects of coexpression of protein 4.2 and three protein-4.2 variants with band 3 in the Xenopus oocyte expression system. Normal protein 4.2 increased band-3-specific chloride transport in the oocytes. Protein 4.2 also coimmunoprecipitated with band 3 and colocalized with band 3 at the oocyte plasma membrane. The increase in band-3-mediated chloride transport and coimmunoprecipitation of protein 4.2 required the presence of the N-terminal cytoplasmic domain of band 3. Protein 4.2 also localized to the oocyte plasma membrane in the absence of band 3. The protein-4.2 variants 4.2 Tozeur (R310Q) and 4.2 Komatsu (D175Y) had impaired ability to bind to band 3 and these variants did not localize to the oocyte plasma membrane when expressed on their own or when coexpressed with band 3. Unexpectedly, 4.2 Nippon (A142T) behaved similarly to normal protein 4.2. In the absence of a crystal structure of protein 4.2, we propose a homology model of protein 4.2 based on the structure of the sequence-related protein transglutaminase. Using our results in oocytes and this homology model we speculate how these mutations affect protein 4.2 and result in hereditary spherocytosis. © 2005 by The American Society of Hematology.
    Original languageEnglish
    Pages (from-to)4088-4095
    Number of pages7
    JournalBlood
    Volume105
    Issue number10
    DOIs
    Publication statusPublished - 15 May 2005

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