Protein Film Electrochemistry of Iron–Sulfur Enzymes

Fraser Armstrong; Rhiannon Evans; Clare Megarity

doi:10.1016/bs.mie.2017.11.001

Protein Film Electrochemistry of Iron–Sulfur Enzymes

Fraser Armstrong, Rhiannon Evans, Clare Megarity

Materials Chemistry

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

Abstract

A suite of dynamic electrochemical techniques known as protein film electrochemistry (PFE) offers important insight into the roles of active sites in enzymes, including properties of electron-transfer centers (individually or collectively), rates and dependences of catalytic electron transport, and binding and dissociation of inhibitors. In this chapter, we explain how PFE is used to investigate the properties of FeS clusters—centers lacking distinctive or convenient spectroscopic signatures that are often very sensitive to O₂. We see that PFE allows simultaneous detection and control of the reactions of individual FeS clusters, and measurement of their relaying efficiency in long-range electron transfer.

Original language	English
Title of host publication	Methods in Methods in Enzymology
Subtitle of host publication	Fe-S Cluster Enzymes Part B
Editors	Sheila S. David
Place of Publication	Cambridge, MA
Chapter	13
Pages	387-407
Number of pages	21
Volume	599
Edition	First
ISBN (Electronic)	9780128147184
DOIs	https://doi.org/10.1016/bs.mie.2017.11.001
Publication status	Published - 20 Jan 2018

Publication series

Name	Methods in Enzymology
Publisher	Academic Press
Volume	599
ISSN (Print)	0076-6879

Access to Document

10.1016/bs.mie.2017.11.001

Cite this

@inbook{5e734e5711044e4ea3286674233258ce,

title = "Protein Film Electrochemistry of Iron–Sulfur Enzymes",

abstract = "A suite of dynamic electrochemical techniques known as protein film electrochemistry (PFE) offers important insight into the roles of active sites in enzymes, including properties of electron-transfer centers (individually or collectively), rates and dependences of catalytic electron transport, and binding and dissociation of inhibitors. In this chapter, we explain how PFE is used to investigate the properties of FeS clusters—centers lacking distinctive or convenient spectroscopic signatures that are often very sensitive to O2. We see that PFE allows simultaneous detection and control of the reactions of individual FeS clusters, and measurement of their relaying efficiency in long-range electron transfer.",

author = "Fraser Armstrong and Rhiannon Evans and Clare Megarity",

year = "2018",

month = jan,

day = "20",

doi = "10.1016/bs.mie.2017.11.001",

language = "English",

isbn = "9780128147177",

volume = "599",

series = "Methods in Enzymology",

publisher = "Academic Press",

pages = "387--407",

editor = "David, {Sheila S.}",

booktitle = "Methods in Methods in Enzymology",

edition = "First",

}

Protein Film Electrochemistry of Iron–Sulfur Enzymes. / Armstrong, Fraser; Evans, Rhiannon; Megarity, Clare.
Methods in Methods in Enzymology: Fe-S Cluster Enzymes Part B. ed. / Sheila S. David. Vol. 599 First. ed. Cambridge, MA, 2018. p. 387-407 (Methods in Enzymology; Vol. 599).

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

TY - CHAP

T1 - Protein Film Electrochemistry of Iron–Sulfur Enzymes

AU - Armstrong, Fraser

AU - Evans, Rhiannon

AU - Megarity, Clare

PY - 2018/1/20

Y1 - 2018/1/20

N2 - A suite of dynamic electrochemical techniques known as protein film electrochemistry (PFE) offers important insight into the roles of active sites in enzymes, including properties of electron-transfer centers (individually or collectively), rates and dependences of catalytic electron transport, and binding and dissociation of inhibitors. In this chapter, we explain how PFE is used to investigate the properties of FeS clusters—centers lacking distinctive or convenient spectroscopic signatures that are often very sensitive to O2. We see that PFE allows simultaneous detection and control of the reactions of individual FeS clusters, and measurement of their relaying efficiency in long-range electron transfer.

AB - A suite of dynamic electrochemical techniques known as protein film electrochemistry (PFE) offers important insight into the roles of active sites in enzymes, including properties of electron-transfer centers (individually or collectively), rates and dependences of catalytic electron transport, and binding and dissociation of inhibitors. In this chapter, we explain how PFE is used to investigate the properties of FeS clusters—centers lacking distinctive or convenient spectroscopic signatures that are often very sensitive to O2. We see that PFE allows simultaneous detection and control of the reactions of individual FeS clusters, and measurement of their relaying efficiency in long-range electron transfer.

UR - http://www.sciencedirect.com/science/article/pii/S0076687917303397

U2 - 10.1016/bs.mie.2017.11.001

DO - 10.1016/bs.mie.2017.11.001

M3 - Chapter

SN - 9780128147177

VL - 599

T3 - Methods in Enzymology

SP - 387

EP - 407

BT - Methods in Methods in Enzymology

A2 - David, Sheila S.

CY - Cambridge, MA

ER -

Protein Film Electrochemistry of Iron–Sulfur Enzymes

Abstract

Publication series

Access to Document

Other files and links

Fingerprint

Cite this