Protein functionalized ZnO thin film bulk acoustic resonator as an odorant biosensor

Xiubo Zhao; Gregory M. Ashley; Luis Garcia-Gancedo; Hao Jin; Jikui Luo; Andrew J. Flewitt; Jian R. Lu

doi:10.1016/j.snb.2012.01.046

Protein functionalized ZnO thin film bulk acoustic resonator as an odorant biosensor

Xiubo Zhao, Gregory M. Ashley, Luis Garcia-Gancedo, Hao Jin, Jikui Luo, Andrew J. Flewitt, Jian R. Lu

Research output: Contribution to journal › Article › peer-review

Abstract

ZnO thin film bulk acoustic resonators (FBARs) with resonant frequency of ∼1.5 GHz have been fabricated to function as an odorant biosensor. Physical adsorption of an odorant binding protein (AaegOBP22 from Aedes aegypti) resulted in frequency down shift. N,N-diethyl-meta-toluamide (DEET) has been selected as a ligand to the odorant binding protein (OBP). Alternate exposure of the bare FBARs to nitrogen flow with and without DEET vapor did not cause any noticeable frequency change. However, frequency drop was detected when exposing the OBP loaded FBAR sensors to the nitrogen flow containing DEET vapor against nitrogen flow alone (control) and the extent of frequency shift was proportional to the amount of the protein immobilized on the FBAR surface, indicating a linear response to DEET binding. These findings demonstrate the potential of binding protein functionalized FBARs as odorant biosensors. © 2012 Elsevier B.V. All rights reserved.

Original language	English
Pages (from-to)	242-246
Number of pages	4
Journal	Sensors and Actuators B: Chemical: international journal devoted to research and development of physical and chemical transducers
Volume	163
Issue number	1
DOIs	https://doi.org/10.1016/j.snb.2012.01.046
Publication status	Published - 1 Mar 2012

Keywords

Biosensor
DEET
FBAR
Gas sensor
Odorant binding protein

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10.1016/j.snb.2012.01.046

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Zhao, X., Ashley, G. M., Garcia-Gancedo, L., Jin, H., Luo, J., Flewitt, A. J., & Lu, J. R. (2012). Protein functionalized ZnO thin film bulk acoustic resonator as an odorant biosensor. Sensors and Actuators B: Chemical: international journal devoted to research and development of physical and chemical transducers, 163(1), 242-246. https://doi.org/10.1016/j.snb.2012.01.046

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title = "Protein functionalized ZnO thin film bulk acoustic resonator as an odorant biosensor",

abstract = "ZnO thin film bulk acoustic resonators (FBARs) with resonant frequency of ∼1.5 GHz have been fabricated to function as an odorant biosensor. Physical adsorption of an odorant binding protein (AaegOBP22 from Aedes aegypti) resulted in frequency down shift. N,N-diethyl-meta-toluamide (DEET) has been selected as a ligand to the odorant binding protein (OBP). Alternate exposure of the bare FBARs to nitrogen flow with and without DEET vapor did not cause any noticeable frequency change. However, frequency drop was detected when exposing the OBP loaded FBAR sensors to the nitrogen flow containing DEET vapor against nitrogen flow alone (control) and the extent of frequency shift was proportional to the amount of the protein immobilized on the FBAR surface, indicating a linear response to DEET binding. These findings demonstrate the potential of binding protein functionalized FBARs as odorant biosensors. {\textcopyright} 2012 Elsevier B.V. All rights reserved.",

keywords = "Biosensor, DEET, FBAR, Gas sensor, Odorant binding protein",

author = "Xiubo Zhao and Ashley, {Gregory M.} and Luis Garcia-Gancedo and Hao Jin and Jikui Luo and Flewitt, {Andrew J.} and Lu, {Jian R.}",

year = "2012",

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doi = "10.1016/j.snb.2012.01.046",

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Zhao, X, Ashley, GM, Garcia-Gancedo, L, Jin, H, Luo, J, Flewitt, AJ & Lu, JR 2012, 'Protein functionalized ZnO thin film bulk acoustic resonator as an odorant biosensor', Sensors and Actuators B: Chemical: international journal devoted to research and development of physical and chemical transducers, vol. 163, no. 1, pp. 242-246. https://doi.org/10.1016/j.snb.2012.01.046

Protein functionalized ZnO thin film bulk acoustic resonator as an odorant biosensor. / Zhao, Xiubo; Ashley, Gregory M.; Garcia-Gancedo, Luis et al.
In: Sensors and Actuators B: Chemical: international journal devoted to research and development of physical and chemical transducers, Vol. 163, No. 1, 01.03.2012, p. 242-246.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Protein functionalized ZnO thin film bulk acoustic resonator as an odorant biosensor

AU - Zhao, Xiubo

AU - Ashley, Gregory M.

AU - Garcia-Gancedo, Luis

AU - Jin, Hao

AU - Luo, Jikui

AU - Flewitt, Andrew J.

AU - Lu, Jian R.

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N2 - ZnO thin film bulk acoustic resonators (FBARs) with resonant frequency of ∼1.5 GHz have been fabricated to function as an odorant biosensor. Physical adsorption of an odorant binding protein (AaegOBP22 from Aedes aegypti) resulted in frequency down shift. N,N-diethyl-meta-toluamide (DEET) has been selected as a ligand to the odorant binding protein (OBP). Alternate exposure of the bare FBARs to nitrogen flow with and without DEET vapor did not cause any noticeable frequency change. However, frequency drop was detected when exposing the OBP loaded FBAR sensors to the nitrogen flow containing DEET vapor against nitrogen flow alone (control) and the extent of frequency shift was proportional to the amount of the protein immobilized on the FBAR surface, indicating a linear response to DEET binding. These findings demonstrate the potential of binding protein functionalized FBARs as odorant biosensors. © 2012 Elsevier B.V. All rights reserved.

AB - ZnO thin film bulk acoustic resonators (FBARs) with resonant frequency of ∼1.5 GHz have been fabricated to function as an odorant biosensor. Physical adsorption of an odorant binding protein (AaegOBP22 from Aedes aegypti) resulted in frequency down shift. N,N-diethyl-meta-toluamide (DEET) has been selected as a ligand to the odorant binding protein (OBP). Alternate exposure of the bare FBARs to nitrogen flow with and without DEET vapor did not cause any noticeable frequency change. However, frequency drop was detected when exposing the OBP loaded FBAR sensors to the nitrogen flow containing DEET vapor against nitrogen flow alone (control) and the extent of frequency shift was proportional to the amount of the protein immobilized on the FBAR surface, indicating a linear response to DEET binding. These findings demonstrate the potential of binding protein functionalized FBARs as odorant biosensors. © 2012 Elsevier B.V. All rights reserved.

KW - Biosensor

KW - DEET

KW - FBAR

KW - Gas sensor

KW - Odorant binding protein

U2 - 10.1016/j.snb.2012.01.046

DO - 10.1016/j.snb.2012.01.046

M3 - Article

SN - 0925-4005

VL - 163

SP - 242

EP - 246

JO - Sensors and Actuators B: Chemical: international journal devoted to research and development of physical and chemical transducers

JF - Sensors and Actuators B: Chemical: international journal devoted to research and development of physical and chemical transducers

IS - 1

ER -

Protein functionalized ZnO thin film bulk acoustic resonator as an odorant biosensor

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Keywords

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