Proteins associated with immunopurified granules from a model pancreatic islet β-Cell system: Proteomic snapshot of an endocrine secretory granule

Anthony J R Hickey; Joshua W I Bradley; Gretchen L. Skea; Martin J. Middleditch; Christina M. Buchanan; Anthony R J Phillips; Garth Cooper

doi:10.1021/pr800675k

Proteins associated with immunopurified granules from a model pancreatic islet β-Cell system: Proteomic snapshot of an endocrine secretory granule

Anthony J R Hickey
, Joshua W I Bradley
, Gretchen L. Skea
, Martin J. Middleditch
, Christina M. Buchanan
, Anthony R J Phillips
, Garth Cooper

Division of Cardiovascular Sciences (L5)

Research output: Contribution to journal › Article › peer-review

Abstract

β-Cell granules contain proteins involved in fuel regulation, which when altered, contribute to metabolic disorders including diabetes mellitus. We analyzed proteins present in purified granules from the INS-IE β-cell model. Fifty-one component proteins were identified by LC-MS/MS including hormones, granins, protein processing components, cellular trafficking components, enzymes implicated in cellular metabolism and chaperone proteins. These findings may increase understanding of granule secretion and the processes leading to protein aggregation and β-cell death in type-2 diabetes. © 2009 American Chemical Society.

Original language	English
Pages (from-to)	178-186
Number of pages	8
Journal	Journal of Proteome Research
Volume	8
Issue number	1
DOIs	https://doi.org/10.1021/pr800675k
Publication status	Published - Jan 2009

Keywords

Chaperone
Diabetes
Immuno-purification
LC-MS/MS
Metabolic disease

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1021/pr800675k

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title = "Proteins associated with immunopurified granules from a model pancreatic islet β-Cell system: Proteomic snapshot of an endocrine secretory granule",

abstract = "β-Cell granules contain proteins involved in fuel regulation, which when altered, contribute to metabolic disorders including diabetes mellitus. We analyzed proteins present in purified granules from the INS-IE β-cell model. Fifty-one component proteins were identified by LC-MS/MS including hormones, granins, protein processing components, cellular trafficking components, enzymes implicated in cellular metabolism and chaperone proteins. These findings may increase understanding of granule secretion and the processes leading to protein aggregation and β-cell death in type-2 diabetes. {\textcopyright} 2009 American Chemical Society.",

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author = "Hickey, \{Anthony J R\} and Bradley, \{Joshua W I\} and Skea, \{Gretchen L.\} and Middleditch, \{Martin J.\} and Buchanan, \{Christina M.\} and Phillips, \{Anthony R J\} and Garth Cooper",

year = "2009",

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AU - Hickey, Anthony J R

AU - Bradley, Joshua W I

AU - Skea, Gretchen L.

AU - Middleditch, Martin J.

AU - Buchanan, Christina M.

AU - Phillips, Anthony R J

AU - Cooper, Garth

PY - 2009/1

Y1 - 2009/1

N2 - β-Cell granules contain proteins involved in fuel regulation, which when altered, contribute to metabolic disorders including diabetes mellitus. We analyzed proteins present in purified granules from the INS-IE β-cell model. Fifty-one component proteins were identified by LC-MS/MS including hormones, granins, protein processing components, cellular trafficking components, enzymes implicated in cellular metabolism and chaperone proteins. These findings may increase understanding of granule secretion and the processes leading to protein aggregation and β-cell death in type-2 diabetes. © 2009 American Chemical Society.

AB - β-Cell granules contain proteins involved in fuel regulation, which when altered, contribute to metabolic disorders including diabetes mellitus. We analyzed proteins present in purified granules from the INS-IE β-cell model. Fifty-one component proteins were identified by LC-MS/MS including hormones, granins, protein processing components, cellular trafficking components, enzymes implicated in cellular metabolism and chaperone proteins. These findings may increase understanding of granule secretion and the processes leading to protein aggregation and β-cell death in type-2 diabetes. © 2009 American Chemical Society.

KW - Chaperone

KW - Diabetes

KW - Immuno-purification

KW - LC-MS/MS

KW - Metabolic disease

U2 - 10.1021/pr800675k

DO - 10.1021/pr800675k

M3 - Article

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JF - Journal of Proteome Research

IS - 1

ER -

Proteins associated with immunopurified granules from a model pancreatic islet β-Cell system: Proteomic snapshot of an endocrine secretory granule

Abstract

Keywords

UN SDGs

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