Proteomic analysis of mouse growth plate cartilage

Daniele Belluoccio, Richard Wilson, David J. Thornton, Tristan P. Wallis, Jeffrey J. Gorman, John F. Bateman

    Research output: Contribution to journalArticlepeer-review


    Cartilage is a highly specialized load-bearing tissue with a small number of cells and a high proportion of extracellular matrix (ECM). The abundance of heavily sulfated proteoglycans and a poorly soluble collagenous ECM presents a major technical challenge to 2-DE. Here we report proteomic analysis of mouse growth plate cartilage using novel methodology for tissue dissection and sample prefractionation. We have successfully resolved cartilage tissue extracts by 2-DE for the first time and identified cartilage ECM proteins by Western blotting and MS/MS. © 2006 Wiley-VCH Verlag GmbH & Co. KGaA.
    Original languageEnglish
    Pages (from-to)6549-6553
    Number of pages4
    Issue number24
    Publication statusPublished - Dec 2006


    • Cartilage
    • Extracellular matrix proteins
    • IEF optimization
    • Prefractionation


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