Proteomic analysis of mouse growth plate cartilage

Daniele Belluoccio; Richard Wilson; David J. Thornton; Tristan P. Wallis; Jeffrey J. Gorman; John F. Bateman

doi:10.1002/pmic.200600191

Proteomic analysis of mouse growth plate cartilage

Daniele Belluoccio, Richard Wilson, David J. Thornton, Tristan P. Wallis, Jeffrey J. Gorman, John F. Bateman

Research output: Contribution to journal › Article › peer-review

Abstract

Cartilage is a highly specialized load-bearing tissue with a small number of cells and a high proportion of extracellular matrix (ECM). The abundance of heavily sulfated proteoglycans and a poorly soluble collagenous ECM presents a major technical challenge to 2-DE. Here we report proteomic analysis of mouse growth plate cartilage using novel methodology for tissue dissection and sample prefractionation. We have successfully resolved cartilage tissue extracts by 2-DE for the first time and identified cartilage ECM proteins by Western blotting and MS/MS. © 2006 Wiley-VCH Verlag GmbH & Co. KGaA.

Original language	English
Pages (from-to)	6549-6553
Number of pages	4
Journal	Proteomics
Volume	6
Issue number	24
DOIs	https://doi.org/10.1002/pmic.200600191
Publication status	Published - Dec 2006

Keywords

Cartilage
Extracellular matrix proteins
IEF optimization
Prefractionation

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10.1002/pmic.200600191

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title = "Proteomic analysis of mouse growth plate cartilage",

abstract = "Cartilage is a highly specialized load-bearing tissue with a small number of cells and a high proportion of extracellular matrix (ECM). The abundance of heavily sulfated proteoglycans and a poorly soluble collagenous ECM presents a major technical challenge to 2-DE. Here we report proteomic analysis of mouse growth plate cartilage using novel methodology for tissue dissection and sample prefractionation. We have successfully resolved cartilage tissue extracts by 2-DE for the first time and identified cartilage ECM proteins by Western blotting and MS/MS. {\textcopyright} 2006 Wiley-VCH Verlag GmbH & Co. KGaA.",

keywords = "Cartilage, Extracellular matrix proteins, IEF optimization, Prefractionation",

author = "Daniele Belluoccio and Richard Wilson and Thornton, {David J.} and Wallis, {Tristan P.} and Gorman, {Jeffrey J.} and Bateman, {John F.}",

year = "2006",

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doi = "10.1002/pmic.200600191",

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AU - Belluoccio, Daniele

AU - Wilson, Richard

AU - Thornton, David J.

AU - Wallis, Tristan P.

AU - Gorman, Jeffrey J.

AU - Bateman, John F.

PY - 2006/12

Y1 - 2006/12

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AB - Cartilage is a highly specialized load-bearing tissue with a small number of cells and a high proportion of extracellular matrix (ECM). The abundance of heavily sulfated proteoglycans and a poorly soluble collagenous ECM presents a major technical challenge to 2-DE. Here we report proteomic analysis of mouse growth plate cartilage using novel methodology for tissue dissection and sample prefractionation. We have successfully resolved cartilage tissue extracts by 2-DE for the first time and identified cartilage ECM proteins by Western blotting and MS/MS. © 2006 Wiley-VCH Verlag GmbH & Co. KGaA.

KW - Cartilage

KW - Extracellular matrix proteins

KW - IEF optimization

KW - Prefractionation

U2 - 10.1002/pmic.200600191

DO - 10.1002/pmic.200600191

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VL - 6

SP - 6549

EP - 6553

JO - Proteomics

JF - Proteomics

IS - 24

ER -

Proteomic analysis of mouse growth plate cartilage

Abstract

Keywords

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