Proteomic analysis reveals that pheophorbide a-mediated photodynamic treatment inhibits prostate cancer growth by hampering GDP-GTP exchange of ras-family proteins

Dan Dan Xu, Chong Bing Xu, Hon Ming Lam, Fuk-ling Wong, Albert Wing Nang Leung, Merrin Man Long Leong, William Chi Shing Cho, Robin Hoeven, Qingtao Lv, Rong Rong

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Background

    We previously reported that pheophorbide a (PhA), excited by 630 nm light, significantly inhibited the growth of prostate cancer cells. In this study, we employed whole-cell proteomics to investigate photodynamic treatment (PDT)-related proteins.

    Methods

    Two-dimensional gel electrophoresis (2-DE) coupled with tandem mass spectrometry was employed to reveal the proteins involved in PhA-mediated PDT in LNCaP and PC-3 prostate cancer cells.

    Results

    After PhA-PDT treatment, decreased expression of translationally-controlled tumor protein (TCTP) was found in both PC-3 and LNCaP whole-cell proteomes. In contrast, human rab GDP dissociation inhibitor (GDI) in LNCaP cells and ras-related homologs GDI in PC-3 cells were up-regulated.

    Conclusions

    GDP-GTP exchange is an underlying target of photodynamic treatment in prostate cancer cells.
    Original languageEnglish
    Pages (from-to)35-39
    JournalPhotodiagnosis and Photodynamic Therapy
    Volume23
    Early online date22 May 2018
    DOIs
    Publication statusPublished - Sep 2018

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