Proteomic analysis reveals that pheophorbide a-mediated photodynamic treatment inhibits prostate cancer growth by hampering GDP-GTP exchange of ras-family proteins

Dan Dan Xu; Chong Bing Xu; Hon Ming Lam; Fuk-ling Wong; Albert Wing Nang Leung; Merrin Man Long Leong; William Chi Shing Cho; Robin Hoeven; Qingtao Lv; Rong Rong

doi:10.1016/j.pdpdt.2018.05.014

Proteomic analysis reveals that pheophorbide a-mediated photodynamic treatment inhibits prostate cancer growth by hampering GDP-GTP exchange of ras-family proteins

Dan Dan Xu, Chong Bing Xu, Hon Ming Lam, Fuk-ling Wong, Albert Wing Nang Leung, Merrin Man Long Leong, William Chi Shing Cho, Robin Hoeven, Qingtao Lv, Rong Rong

Research output: Contribution to journal › Article › peer-review

Abstract

Background

We previously reported that pheophorbide a (PhA), excited by 630 nm light, significantly inhibited the growth of prostate cancer cells. In this study, we employed whole-cell proteomics to investigate photodynamic treatment (PDT)-related proteins.

Methods

Two-dimensional gel electrophoresis (2-DE) coupled with tandem mass spectrometry was employed to reveal the proteins involved in PhA-mediated PDT in LNCaP and PC-3 prostate cancer cells.

Results

After PhA-PDT treatment, decreased expression of translationally-controlled tumor protein (TCTP) was found in both PC-3 and LNCaP whole-cell proteomes. In contrast, human rab GDP dissociation inhibitor (GDI) in LNCaP cells and ras-related homologs GDI in PC-3 cells were up-regulated.

Conclusions

GDP-GTP exchange is an underlying target of photodynamic treatment in prostate cancer cells.

Original language	English
Pages (from-to)	35-39
Journal	Photodiagnosis and Photodynamic Therapy
Volume	23
Early online date	22 May 2018
DOIs	https://doi.org/10.1016/j.pdpdt.2018.05.014
Publication status	Published - Sept 2018

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Xu, D. D., Xu, C. B., Lam, H. M., Wong, F., Leung, A. W. N., Leong, M. M. L., Cho, W. C. S., Hoeven, R., Lv, Q., & Rong, R. (2018). Proteomic analysis reveals that pheophorbide a-mediated photodynamic treatment inhibits prostate cancer growth by hampering GDP-GTP exchange of ras-family proteins. Photodiagnosis and Photodynamic Therapy, 23, 35-39. https://doi.org/10.1016/j.pdpdt.2018.05.014

@article{aecd9a60d96d4eb59ad7e1f5faa84fb2,

title = "Proteomic analysis reveals that pheophorbide a-mediated photodynamic treatment inhibits prostate cancer growth by hampering GDP-GTP exchange of ras-family proteins",

abstract = "BackgroundWe previously reported that pheophorbide a (PhA), excited by 630 nm light, significantly inhibited the growth of prostate cancer cells. In this study, we employed whole-cell proteomics to investigate photodynamic treatment (PDT)-related proteins.MethodsTwo-dimensional gel electrophoresis (2-DE) coupled with tandem mass spectrometry was employed to reveal the proteins involved in PhA-mediated PDT in LNCaP and PC-3 prostate cancer cells.ResultsAfter PhA-PDT treatment, decreased expression of translationally-controlled tumor protein (TCTP) was found in both PC-3 and LNCaP whole-cell proteomes. In contrast, human rab GDP dissociation inhibitor (GDI) in LNCaP cells and ras-related homologs GDI in PC-3 cells were up-regulated.ConclusionsGDP-GTP exchange is an underlying target of photodynamic treatment in prostate cancer cells.",

author = "Xu, {Dan Dan} and Xu, {Chong Bing} and Lam, {Hon Ming} and Fuk-ling Wong and Leung, {Albert Wing Nang} and Leong, {Merrin Man Long} and Cho, {William Chi Shing} and Robin Hoeven and Qingtao Lv and Rong Rong",

year = "2018",

month = sep,

doi = "10.1016/j.pdpdt.2018.05.014",

language = "English",

volume = "23",

pages = "35--39",

journal = "Photodiagnosis and Photodynamic Therapy",

issn = "1572-1000",

publisher = "Elsevier BV",

}

Xu, DD, Xu, CB, Lam, HM, Wong, F, Leung, AWN, Leong, MML, Cho, WCS, Hoeven, R, Lv, Q & Rong, R 2018, 'Proteomic analysis reveals that pheophorbide a-mediated photodynamic treatment inhibits prostate cancer growth by hampering GDP-GTP exchange of ras-family proteins', Photodiagnosis and Photodynamic Therapy, vol. 23, pp. 35-39. https://doi.org/10.1016/j.pdpdt.2018.05.014

TY - JOUR

T1 - Proteomic analysis reveals that pheophorbide a-mediated photodynamic treatment inhibits prostate cancer growth by hampering GDP-GTP exchange of ras-family proteins

AU - Xu, Dan Dan

AU - Xu, Chong Bing

AU - Lam, Hon Ming

AU - Wong, Fuk-ling

AU - Leung, Albert Wing Nang

AU - Leong, Merrin Man Long

AU - Cho, William Chi Shing

AU - Hoeven, Robin

AU - Lv, Qingtao

AU - Rong, Rong

PY - 2018/9

Y1 - 2018/9

N2 - BackgroundWe previously reported that pheophorbide a (PhA), excited by 630 nm light, significantly inhibited the growth of prostate cancer cells. In this study, we employed whole-cell proteomics to investigate photodynamic treatment (PDT)-related proteins.MethodsTwo-dimensional gel electrophoresis (2-DE) coupled with tandem mass spectrometry was employed to reveal the proteins involved in PhA-mediated PDT in LNCaP and PC-3 prostate cancer cells.ResultsAfter PhA-PDT treatment, decreased expression of translationally-controlled tumor protein (TCTP) was found in both PC-3 and LNCaP whole-cell proteomes. In contrast, human rab GDP dissociation inhibitor (GDI) in LNCaP cells and ras-related homologs GDI in PC-3 cells were up-regulated.ConclusionsGDP-GTP exchange is an underlying target of photodynamic treatment in prostate cancer cells.

AB - BackgroundWe previously reported that pheophorbide a (PhA), excited by 630 nm light, significantly inhibited the growth of prostate cancer cells. In this study, we employed whole-cell proteomics to investigate photodynamic treatment (PDT)-related proteins.MethodsTwo-dimensional gel electrophoresis (2-DE) coupled with tandem mass spectrometry was employed to reveal the proteins involved in PhA-mediated PDT in LNCaP and PC-3 prostate cancer cells.ResultsAfter PhA-PDT treatment, decreased expression of translationally-controlled tumor protein (TCTP) was found in both PC-3 and LNCaP whole-cell proteomes. In contrast, human rab GDP dissociation inhibitor (GDI) in LNCaP cells and ras-related homologs GDI in PC-3 cells were up-regulated.ConclusionsGDP-GTP exchange is an underlying target of photodynamic treatment in prostate cancer cells.

U2 - 10.1016/j.pdpdt.2018.05.014

DO - 10.1016/j.pdpdt.2018.05.014

M3 - Article

SN - 1572-1000

VL - 23

SP - 35

EP - 39

JO - Photodiagnosis and Photodynamic Therapy

JF - Photodiagnosis and Photodynamic Therapy

ER -

Proteomic analysis reveals that pheophorbide a-mediated photodynamic treatment inhibits prostate cancer growth by hampering GDP-GTP exchange of ras-family proteins

Abstract

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