Protocol for live-cell imaging during Tumor Treating Fields treatment with Inovitro Live

Paul L G Slangen; Yaara Porat; Marjolijn Mertz; Bram van den Broek; Kees Jalink; Mark C de Gooijer; Olaf van Tellingen; Gerben R Borst

doi:10.1016/j.xpro.2022.101246

Protocol for live-cell imaging during Tumor Treating Fields treatment with Inovitro Live

Paul L G Slangen, Yaara Porat, Marjolijn Mertz, Bram van den Broek, Kees Jalink, Mark C de Gooijer, Olaf van Tellingen, Gerben R Borst

Division of Cancer Sciences (L5)

Research output: Contribution to journal › Article › peer-review

Abstract

Tumor Treating Fields (TTFields) are an FDA-approved anticancer treatment using alternating electric fields. Here, we present a protocol to perform live-cell imaging (LCI) of cells during TTFields treatment with the Inovitro LiveTM system. The setup we describe dissipates TTFields-related heat production and can be used in conjunction with any LCI-compatible microscope setup. This approach will enable further elucidation of TTFields' mechanism of action at the molecular level and facilitate the development of promising combination strategies.

Original language	English
Pages (from-to)	101246
Journal	STAR protocols
Volume	3
Issue number	2
DOIs	https://doi.org/10.1016/j.xpro.2022.101246
Publication status	Published - 17 Jun 2022

Keywords

Combined Modality Therapy
Electric Stimulation Therapy/methods
Humans
Neoplasms/diagnostic imaging

Research Beacons, Institutes and Platforms

Manchester Cancer Research Centre

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.xpro.2022.101246

Cite this

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title = "Protocol for live-cell imaging during Tumor Treating Fields treatment with Inovitro Live",

abstract = "Tumor Treating Fields (TTFields) are an FDA-approved anticancer treatment using alternating electric fields. Here, we present a protocol to perform live-cell imaging (LCI) of cells during TTFields treatment with the Inovitro LiveTM system. The setup we describe dissipates TTFields-related heat production and can be used in conjunction with any LCI-compatible microscope setup. This approach will enable further elucidation of TTFields' mechanism of action at the molecular level and facilitate the development of promising combination strategies.",

keywords = "Combined Modality Therapy, Electric Stimulation Therapy/methods, Humans, Neoplasms/diagnostic imaging",

author = "Slangen, {Paul L G} and Yaara Porat and Marjolijn Mertz and {van den Broek}, Bram and Kees Jalink and {de Gooijer}, {Mark C} and {van Tellingen}, Olaf and Borst, {Gerben R}",

note = "{\textcopyright} 2022 The Authors.",

year = "2022",

month = jun,

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doi = "10.1016/j.xpro.2022.101246",

language = "English",

volume = "3",

pages = "101246",

journal = "STAR protocols",

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TY - JOUR

T1 - Protocol for live-cell imaging during Tumor Treating Fields treatment with Inovitro Live

AU - Slangen, Paul L G

AU - Porat, Yaara

AU - Mertz, Marjolijn

AU - van den Broek, Bram

AU - Jalink, Kees

AU - de Gooijer, Mark C

AU - van Tellingen, Olaf

AU - Borst, Gerben R

PY - 2022/6/17

Y1 - 2022/6/17

N2 - Tumor Treating Fields (TTFields) are an FDA-approved anticancer treatment using alternating electric fields. Here, we present a protocol to perform live-cell imaging (LCI) of cells during TTFields treatment with the Inovitro LiveTM system. The setup we describe dissipates TTFields-related heat production and can be used in conjunction with any LCI-compatible microscope setup. This approach will enable further elucidation of TTFields' mechanism of action at the molecular level and facilitate the development of promising combination strategies.

AB - Tumor Treating Fields (TTFields) are an FDA-approved anticancer treatment using alternating electric fields. Here, we present a protocol to perform live-cell imaging (LCI) of cells during TTFields treatment with the Inovitro LiveTM system. The setup we describe dissipates TTFields-related heat production and can be used in conjunction with any LCI-compatible microscope setup. This approach will enable further elucidation of TTFields' mechanism of action at the molecular level and facilitate the development of promising combination strategies.

KW - Combined Modality Therapy

KW - Electric Stimulation Therapy/methods

KW - Humans

KW - Neoplasms/diagnostic imaging

U2 - 10.1016/j.xpro.2022.101246

DO - 10.1016/j.xpro.2022.101246

M3 - Article

C2 - 35368806

SN - 2666-1667

VL - 3

SP - 101246

JO - STAR protocols

JF - STAR protocols

IS - 2

ER -

Protocol for live-cell imaging during Tumor Treating Fields treatment with Inovitro Live

Abstract

Keywords

Research Beacons, Institutes and Platforms

UN SDGs

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