Protocol for live-cell imaging during Tumor Treating Fields treatment with Inovitro Live

Paul L G Slangen, Yaara Porat, Marjolijn Mertz, Bram van den Broek, Kees Jalink, Mark C de Gooijer, Olaf van Tellingen, Gerben R Borst

Research output: Contribution to journalArticlepeer-review

Abstract

Tumor Treating Fields (TTFields) are an FDA-approved anticancer treatment using alternating electric fields. Here, we present a protocol to perform live-cell imaging (LCI) of cells during TTFields treatment with the Inovitro LiveTM system. The setup we describe dissipates TTFields-related heat production and can be used in conjunction with any LCI-compatible microscope setup. This approach will enable further elucidation of TTFields' mechanism of action at the molecular level and facilitate the development of promising combination strategies.

Original languageEnglish
Pages (from-to)101246
JournalSTAR protocols
Volume3
Issue number2
DOIs
Publication statusPublished - 17 Jun 2022

Keywords

  • Combined Modality Therapy
  • Electric Stimulation Therapy/methods
  • Humans
  • Neoplasms/diagnostic imaging

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