Purification, characterization and sequence determination of BmKK4, a novel potassium channel blocker from Chinese scorpion Buthus martensi Karsch

Naixia Zhang; Gong Wu; Houming Wu; Michael J. Chalmers; Simon J. Gaskell

doi:10.1016/j.peptides.2004.03.014

Purification, characterization and sequence determination of BmKK4, a novel potassium channel blocker from Chinese scorpion Buthus martensi Karsch

Naixia Zhang, Gong Wu, Houming Wu, Michael J. Chalmers, Simon J. Gaskell

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Abstract

The scorpion neurotoxin BmKK4 was purified from the venom of the Chinese scorpion Buthus martensi Karsch by a combination of gel-filtration, ion exchange and reversed phase chromatography. The primary sequence of BmKK4 was determined using the tandem MS/MS technique and the cDNA database searching as followings: ZTQCQ SVRDC QQYCL TPDRC SYGTC YCKTT (NH2). BmKK4 is the first isolated member of a new subfamily α-KTx17 of scorpion K+ toxins. © 2004 Elsevier Inc. All rights reserved.

Original language	English
Pages (from-to)	951-957
Number of pages	6
Journal	Peptides
Volume	25
Issue number	6
DOIs	https://doi.org/10.1016/j.peptides.2004.03.014
Publication status	Published - Jun 2004

Keywords

BmKK4
Buthus martensi Karsch
Potassium channel blocker, primary sequence
Scorpion toxin
Tandem CID MS/MS

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10.1016/j.peptides.2004.03.014

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@article{1427bd00d897463cb815c428fa2500a7,

title = "Purification, characterization and sequence determination of BmKK4, a novel potassium channel blocker from Chinese scorpion Buthus martensi Karsch",

abstract = "The scorpion neurotoxin BmKK4 was purified from the venom of the Chinese scorpion Buthus martensi Karsch by a combination of gel-filtration, ion exchange and reversed phase chromatography. The primary sequence of BmKK4 was determined using the tandem MS/MS technique and the cDNA database searching as followings: ZTQCQ SVRDC QQYCL TPDRC SYGTC YCKTT (NH2). BmKK4 is the first isolated member of a new subfamily α-KTx17 of scorpion K+ toxins. {\textcopyright} 2004 Elsevier Inc. All rights reserved.",

keywords = "BmKK4, Buthus martensi Karsch, Potassium channel blocker, primary sequence, Scorpion toxin, Tandem CID MS/MS",

author = "Naixia Zhang and Gong Wu and Houming Wu and Chalmers, {Michael J.} and Gaskell, {Simon J.}",

year = "2004",

month = jun,

doi = "10.1016/j.peptides.2004.03.014",

language = "English",

volume = "25",

pages = "951--957",

journal = "Peptides",

issn = "1873-5169",

publisher = "Elsevier BV",

number = "6",

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TY - JOUR

T1 - Purification, characterization and sequence determination of BmKK4, a novel potassium channel blocker from Chinese scorpion Buthus martensi Karsch

AU - Zhang, Naixia

AU - Wu, Gong

AU - Wu, Houming

AU - Chalmers, Michael J.

AU - Gaskell, Simon J.

PY - 2004/6

Y1 - 2004/6

N2 - The scorpion neurotoxin BmKK4 was purified from the venom of the Chinese scorpion Buthus martensi Karsch by a combination of gel-filtration, ion exchange and reversed phase chromatography. The primary sequence of BmKK4 was determined using the tandem MS/MS technique and the cDNA database searching as followings: ZTQCQ SVRDC QQYCL TPDRC SYGTC YCKTT (NH2). BmKK4 is the first isolated member of a new subfamily α-KTx17 of scorpion K+ toxins. © 2004 Elsevier Inc. All rights reserved.

AB - The scorpion neurotoxin BmKK4 was purified from the venom of the Chinese scorpion Buthus martensi Karsch by a combination of gel-filtration, ion exchange and reversed phase chromatography. The primary sequence of BmKK4 was determined using the tandem MS/MS technique and the cDNA database searching as followings: ZTQCQ SVRDC QQYCL TPDRC SYGTC YCKTT (NH2). BmKK4 is the first isolated member of a new subfamily α-KTx17 of scorpion K+ toxins. © 2004 Elsevier Inc. All rights reserved.

KW - BmKK4

KW - Buthus martensi Karsch

KW - Potassium channel blocker, primary sequence

KW - Scorpion toxin

KW - Tandem CID MS/MS

U2 - 10.1016/j.peptides.2004.03.014

DO - 10.1016/j.peptides.2004.03.014

M3 - Article

SN - 1873-5169

VL - 25

SP - 951

EP - 957

JO - Peptides

JF - Peptides

IS - 6

ER -

Purification, characterization and sequence determination of BmKK4, a novel potassium channel blocker from Chinese scorpion Buthus martensi Karsch

Abstract

Keywords

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