Quantal release of ATP in mouse cortex

Yuriy Pankratov, Ulyana Lalo, Alexei Verkhratsky, R. Alan North

Research output: Contribution to journalArticlepeer-review

Abstract

Transient currents occur at rest in cortical neurones that reflect the quantal release of transmitters such as glutamate and γ-aminobutyric acid (GABA). We found a bimodal amplitude distribution for spontaneously occurring inward currents recorded from mouse pyramidal neurones in situ, in acutely isolated brain slices superfused with picrotoxin. Larger events were blocked by glutamate receptor (AMPA, kainate) antagonists; smaller events were partially inhibited by P2X receptor antagonists suramin and PPADS. The decay of the larger events was selectively prolonged by cyclothiazide. Stimulation of single intracortical axons elicited quantal glutamate-mediated currents and also quantal currents with amplitudes corresponding to the smaller spontaneous inward currents. It is likely that the lower amplitude spontaneous events reflect packaged ATP release. This occurs with a lower probability than that of glutamate, and evokes unitary currents about half the amplitude of those mediated through AMPA receptors. Furthermore, the packets of ATP appear to be released from vesicle in a subset of glutamate-containing terminals. © The Rockefeller University Press.
Original languageEnglish
Pages (from-to)257-265
Number of pages8
JournalJournal of General Physiology
Volume129
Issue number3
DOIs
Publication statusPublished - Mar 2007

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