TY - JOUR
T1 - Quantitative online liquid chromatography-surface-enhanced Raman scattering of purine bases
AU - Cowcher, David P.
AU - Jarvis, Roger
AU - Goodacre, Royston
PY - 2014/10/7
Y1 - 2014/10/7
N2 - Raman spectroscopy has been of interest as a detection method for liquid chromatographic separations for a significant period of time, due to the structural information it can provide, allowing the identification and distinction of coeluting analytes. Combined with the rapidly advancing field of enhanced Raman techniques, such as surface-enhanced Raman scattering (SERS), the previous low sensitivity of Raman measurements has also been alleviated. At-line LC-SERS analyses, where SERS measurements are taken of fractions collected during or after HPLC separation have been shown to be sensitive and applicable to a wide variety of analytes; however, quantitative, real-time, online LC-SERS analysis at comparable sensitivity to existing methods, applicable to high-throughput experiments, has not been previously demonstrated. Here we show that by introducing silver colloid, followed by an aggregating agent into the postcolumn flow of an HPLC system, we can quantitatively and reproducibly analyze mixtures of purine bases, with limits of detection in the region of 100-500 pmol. The analysis is performed without the use of a flow cell, thereby eliminating previously detrimental memory effects.
AB - Raman spectroscopy has been of interest as a detection method for liquid chromatographic separations for a significant period of time, due to the structural information it can provide, allowing the identification and distinction of coeluting analytes. Combined with the rapidly advancing field of enhanced Raman techniques, such as surface-enhanced Raman scattering (SERS), the previous low sensitivity of Raman measurements has also been alleviated. At-line LC-SERS analyses, where SERS measurements are taken of fractions collected during or after HPLC separation have been shown to be sensitive and applicable to a wide variety of analytes; however, quantitative, real-time, online LC-SERS analysis at comparable sensitivity to existing methods, applicable to high-throughput experiments, has not been previously demonstrated. Here we show that by introducing silver colloid, followed by an aggregating agent into the postcolumn flow of an HPLC system, we can quantitatively and reproducibly analyze mixtures of purine bases, with limits of detection in the region of 100-500 pmol. The analysis is performed without the use of a flow cell, thereby eliminating previously detrimental memory effects.
UR - http://www.scopus.com/inward/record.url?scp=84907906960&partnerID=8YFLogxK
U2 - 10.1021/ac5029159
DO - 10.1021/ac5029159
M3 - Article
C2 - 25196415
AN - SCOPUS:84907906960
SN - 0003-2700
VL - 86
SP - 9977
EP - 9984
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 19
ER -