Quantitative preservation of viability of aspergillus fumigatus

Quantitative preservation of the viability of two isolates of Aspergillus fumigatus was studied over a 15-month period of storage. Significant loss of viability occurred in isolates preserved at -70°C in both phosphate-buffered saline with Tween (PBST) and 0.1% gelatin, immediately, and between 6 and 15 months. Storage in 10% and 25% dimethyl sulphoxide at -70°C was successful up to 8 weeks with gradual loss of viability later. PBST at 4°C or room temperature, or 10% glycerol at -70°C maintained 100% viability of the cultures up to 6 months and 15 months, respectively. To preserve 100% viability, long-term storage of A. fumigatus is best done in 10% glycerol in PBST at -70°C compared with the other methods tested.