Quantum dot FRET biosensors that respond to pH, to proteolytic or nucleolytic cleavage, to DNA synthesis, or to a multiplexing combination

Miho Suzuki, Yuzuru Husimi, Hirokazu Komatsu, Koji Suzuki, Kenneth T. Douglas

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Fluorescent acceptors have been immobilized on nanoparticulate quantum dots (QDs), which serve in turn as their FRET donors. The broad excitation and narrow emission bands of QDs mark them as having excellent potential as donors for FRET and, in principle, differently colored QDs could be excited simultaneously. The present work describes the preparation and operation of FRET-based QD bioprobes individually able to detect the actions of protease, deoxyribonuclease, DNA polymerase, or changes in pH. In addition, two such QD-mounted biosensors were excited at a single wavelength, and shown to operate simultaneously and independently of each other in the same sample solution, allowing multiplex detection of the action of a protease, trypsin, in the presence of deoxyribonuclease. © 2008 American Chemical Society.
    Original languageEnglish
    Pages (from-to)5720-5725
    Number of pages5
    JournalJournal of the American Chemical Society
    Volume130
    Issue number17
    DOIs
    Publication statusPublished - 30 Apr 2008

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