TY - JOUR
T1 - Quantum dot FRET biosensors that respond to pH, to proteolytic or nucleolytic cleavage, to DNA synthesis, or to a multiplexing combination
AU - Suzuki, Miho
AU - Husimi, Yuzuru
AU - Komatsu, Hirokazu
AU - Suzuki, Koji
AU - Douglas, Kenneth T.
PY - 2008/4/30
Y1 - 2008/4/30
N2 - Fluorescent acceptors have been immobilized on nanoparticulate quantum dots (QDs), which serve in turn as their FRET donors. The broad excitation and narrow emission bands of QDs mark them as having excellent potential as donors for FRET and, in principle, differently colored QDs could be excited simultaneously. The present work describes the preparation and operation of FRET-based QD bioprobes individually able to detect the actions of protease, deoxyribonuclease, DNA polymerase, or changes in pH. In addition, two such QD-mounted biosensors were excited at a single wavelength, and shown to operate simultaneously and independently of each other in the same sample solution, allowing multiplex detection of the action of a protease, trypsin, in the presence of deoxyribonuclease. © 2008 American Chemical Society.
AB - Fluorescent acceptors have been immobilized on nanoparticulate quantum dots (QDs), which serve in turn as their FRET donors. The broad excitation and narrow emission bands of QDs mark them as having excellent potential as donors for FRET and, in principle, differently colored QDs could be excited simultaneously. The present work describes the preparation and operation of FRET-based QD bioprobes individually able to detect the actions of protease, deoxyribonuclease, DNA polymerase, or changes in pH. In addition, two such QD-mounted biosensors were excited at a single wavelength, and shown to operate simultaneously and independently of each other in the same sample solution, allowing multiplex detection of the action of a protease, trypsin, in the presence of deoxyribonuclease. © 2008 American Chemical Society.
U2 - 10.1021/ja710870e
DO - 10.1021/ja710870e
M3 - Article
SN - 0002-7863
VL - 130
SP - 5720
EP - 5725
JO - Journal of the American Chemical Society
JF - Journal of the American Chemical Society
IS - 17
ER -