Rab25 associates with α5β1 integrin to promote invasive migration in 3D microenvironments

Patrick T. Caswell; Heather J. Spence; Maddy Parsons; Dominic P. White; Katherine Clark; Kwai Wa Cheng; Gordon B. Mills; Martin J. Humphries; Anthea J. Messent; Kurt I. Anderson; Mary W. McCaffrey; Bradford W. Ozanne; Jim C. Norman

doi:10.1016/j.devcel.2007.08.012

Rab25 associates with α5β1 integrin to promote invasive migration in 3D microenvironments

Patrick T. Caswell, Heather J. Spence, Maddy Parsons, Dominic P. White, Katherine Clark, Kwai Wa Cheng, Gordon B. Mills, Martin J. Humphries, Anthea J. Messent, Kurt I. Anderson, Mary W. McCaffrey, Bradford W. Ozanne, Jim C. Norman

Research output: Contribution to journal › Article › peer-review

Abstract

Here, we report a direct interaction between the β1 integrin cytoplasmic tail and Rab25, a GTPase that has been linked to tumor aggressiveness and metastasis. Rab25 promotes a mode of migration on 3D matrices that is characterized by the extension of long pseudopodia, and the association of the GTPase with α5β1 promotes localization of vesicles that deliver integrin to the plasma membrane at pseudopodial tips as well as the retention of a pool of cycling α5β1 at the cell front. Furthermore, Rab25-driven tumor-cell invasion into a 3D extracellular matrix environment is strongly dependent on ligation of fibronectin by α5β1 integrin and the capacity of Rab25 to interact with β1 integrin. These data indicate that Rab25 contributes to tumor progression by directing the localization of integrin-recycling vesicles and thereby enhancing the ability of tumor cells to invade the extracellular matrix. © 2007 Elsevier Inc. All rights reserved.

Original language	English
Pages (from-to)	496-510
Number of pages	14
Journal	Developmental cell
Volume	13
Issue number	4
DOIs	https://doi.org/10.1016/j.devcel.2007.08.012
Publication status	Published - 9 Oct 2007

Keywords

CELLBIO

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.devcel.2007.08.012

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Caswell, P. T., Spence, H. J., Parsons, M., White, D. P., Clark, K., Cheng, K. W., Mills, G. B., Humphries, M. J., Messent, A. J., Anderson, K. I., McCaffrey, M. W., Ozanne, B. W., & Norman, J. C. (2007). Rab25 associates with α5β1 integrin to promote invasive migration in 3D microenvironments. Developmental cell, 13(4), 496-510. https://doi.org/10.1016/j.devcel.2007.08.012

@article{656b76cc133f4fd8853fa6e41c2c68ee,

title = "Rab25 associates with α5β1 integrin to promote invasive migration in 3D microenvironments",

abstract = "Here, we report a direct interaction between the β1 integrin cytoplasmic tail and Rab25, a GTPase that has been linked to tumor aggressiveness and metastasis. Rab25 promotes a mode of migration on 3D matrices that is characterized by the extension of long pseudopodia, and the association of the GTPase with α5β1 promotes localization of vesicles that deliver integrin to the plasma membrane at pseudopodial tips as well as the retention of a pool of cycling α5β1 at the cell front. Furthermore, Rab25-driven tumor-cell invasion into a 3D extracellular matrix environment is strongly dependent on ligation of fibronectin by α5β1 integrin and the capacity of Rab25 to interact with β1 integrin. These data indicate that Rab25 contributes to tumor progression by directing the localization of integrin-recycling vesicles and thereby enhancing the ability of tumor cells to invade the extracellular matrix. {\textcopyright} 2007 Elsevier Inc. All rights reserved.",

keywords = "CELLBIO",

author = "Caswell, {Patrick T.} and Spence, {Heather J.} and Maddy Parsons and White, {Dominic P.} and Katherine Clark and Cheng, {Kwai Wa} and Mills, {Gordon B.} and Humphries, {Martin J.} and Messent, {Anthea J.} and Anderson, {Kurt I.} and McCaffrey, {Mary W.} and Ozanne, {Bradford W.} and Norman, {Jim C.}",

year = "2007",

month = oct,

day = "9",

doi = "10.1016/j.devcel.2007.08.012",

language = "English",

volume = "13",

pages = "496--510",

journal = "Developmental cell",

issn = "1878-1551",

publisher = "Cell Press",

number = "4",

}

TY - JOUR

T1 - Rab25 associates with α5β1 integrin to promote invasive migration in 3D microenvironments

AU - Caswell, Patrick T.

AU - Spence, Heather J.

AU - Parsons, Maddy

AU - White, Dominic P.

AU - Clark, Katherine

AU - Cheng, Kwai Wa

AU - Mills, Gordon B.

AU - Humphries, Martin J.

AU - Messent, Anthea J.

AU - Anderson, Kurt I.

AU - McCaffrey, Mary W.

AU - Ozanne, Bradford W.

AU - Norman, Jim C.

PY - 2007/10/9

Y1 - 2007/10/9

N2 - Here, we report a direct interaction between the β1 integrin cytoplasmic tail and Rab25, a GTPase that has been linked to tumor aggressiveness and metastasis. Rab25 promotes a mode of migration on 3D matrices that is characterized by the extension of long pseudopodia, and the association of the GTPase with α5β1 promotes localization of vesicles that deliver integrin to the plasma membrane at pseudopodial tips as well as the retention of a pool of cycling α5β1 at the cell front. Furthermore, Rab25-driven tumor-cell invasion into a 3D extracellular matrix environment is strongly dependent on ligation of fibronectin by α5β1 integrin and the capacity of Rab25 to interact with β1 integrin. These data indicate that Rab25 contributes to tumor progression by directing the localization of integrin-recycling vesicles and thereby enhancing the ability of tumor cells to invade the extracellular matrix. © 2007 Elsevier Inc. All rights reserved.

AB - Here, we report a direct interaction between the β1 integrin cytoplasmic tail and Rab25, a GTPase that has been linked to tumor aggressiveness and metastasis. Rab25 promotes a mode of migration on 3D matrices that is characterized by the extension of long pseudopodia, and the association of the GTPase with α5β1 promotes localization of vesicles that deliver integrin to the plasma membrane at pseudopodial tips as well as the retention of a pool of cycling α5β1 at the cell front. Furthermore, Rab25-driven tumor-cell invasion into a 3D extracellular matrix environment is strongly dependent on ligation of fibronectin by α5β1 integrin and the capacity of Rab25 to interact with β1 integrin. These data indicate that Rab25 contributes to tumor progression by directing the localization of integrin-recycling vesicles and thereby enhancing the ability of tumor cells to invade the extracellular matrix. © 2007 Elsevier Inc. All rights reserved.

KW - CELLBIO

U2 - 10.1016/j.devcel.2007.08.012

DO - 10.1016/j.devcel.2007.08.012

M3 - Article

C2 - 17925226

SN - 1878-1551

VL - 13

SP - 496

EP - 510

JO - Developmental cell

JF - Developmental cell

IS - 4

ER -

Rab25 associates with α5β1 integrin to promote invasive migration in 3D microenvironments

Abstract

Keywords

UN SDGs

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