Rapid and sensitive monitoring of biocatalytic reactions using ion mobility mass spectrometry

Cunyu Yan; Jason Schmidberger; Fabio Parmeggiani; Shaneela Hussain; Nicholas Turner; Sabine Flitsch; Perdita Barran

doi:10.1039/C6AN00617E

Rapid and sensitive monitoring of biocatalytic reactions using ion mobility mass spectrometry

Cunyu Yan, Jason Schmidberger, Fabio Parmeggiani, Shaneela Hussain, Nicholas Turner, Sabine Flitsch, Perdita Barran

Research output: Contribution to journal › Article › peer-review

Abstract

The combination of stable isotope labelling with direct infusion ion mobility mass spectrometry (IM-MS) enabled qualitative and quantitative monitoring of biocatalytic reactions with reduced analysis times, enhanced sensitivity and μL-level assay volumes. The new approach was demonstrated by applying to both lipase and monooxygenase enzymes, including multi-substrate screening.

Original language	English
Journal	Analyst
Early online date	16 Mar 2016
DOIs	https://doi.org/10.1039/C6AN00617E
Publication status	Published - 2016

Research Beacons, Institutes and Platforms

Manchester Institute of Biotechnology

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10.1039/C6AN00617E

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title = "Rapid and sensitive monitoring of biocatalytic reactions using ion mobility mass spectrometry",

abstract = "The combination of stable isotope labelling with direct infusion ion mobility mass spectrometry (IM-MS) enabled qualitative and quantitative monitoring of biocatalytic reactions with reduced analysis times, enhanced sensitivity and μL-level assay volumes. The new approach was demonstrated by applying to both lipase and monooxygenase enzymes, including multi-substrate screening.",

author = "Cunyu Yan and Jason Schmidberger and Fabio Parmeggiani and Shaneela Hussain and Nicholas Turner and Sabine Flitsch and Perdita Barran",

year = "2016",

doi = "10.1039/C6AN00617E",

language = "English",

journal = "Analyst",

issn = "0003-2654",

publisher = "Royal Society of Chemistry",

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T1 - Rapid and sensitive monitoring of biocatalytic reactions using ion mobility mass spectrometry

AU - Yan, Cunyu

AU - Schmidberger, Jason

AU - Parmeggiani, Fabio

AU - Hussain, Shaneela

AU - Turner, Nicholas

AU - Flitsch, Sabine

AU - Barran, Perdita

PY - 2016

Y1 - 2016

N2 - The combination of stable isotope labelling with direct infusion ion mobility mass spectrometry (IM-MS) enabled qualitative and quantitative monitoring of biocatalytic reactions with reduced analysis times, enhanced sensitivity and μL-level assay volumes. The new approach was demonstrated by applying to both lipase and monooxygenase enzymes, including multi-substrate screening.

AB - The combination of stable isotope labelling with direct infusion ion mobility mass spectrometry (IM-MS) enabled qualitative and quantitative monitoring of biocatalytic reactions with reduced analysis times, enhanced sensitivity and μL-level assay volumes. The new approach was demonstrated by applying to both lipase and monooxygenase enzymes, including multi-substrate screening.

U2 - 10.1039/C6AN00617E

DO - 10.1039/C6AN00617E

M3 - Article

SN - 0003-2654

JO - Analyst

JF - Analyst

ER -

Rapid and sensitive monitoring of biocatalytic reactions using ion mobility mass spectrometry

Abstract

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Manchester Synthetic Biology Research Centre for Fine and Speciality Chemicals

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Rapid and sensitive monitoring of biocatalytic reactions using ion mobility mass spectrometry

Abstract

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Manchester Synthetic Biology Research Centre for Fine and Speciality Chemicals

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