Rapid enzyme-linked immunosorbent assay (ELISA) for Aspergillus fumigatus antibodies

M. D. Richardson, J. M. Stubbins, D. W. Warnock

    Research output: Contribution to journalArticlepeer-review


    A rapid enzyme-linked immunosorbent assay (ELISA) where component incubation periods were shortened to one hour, was compared with agar gel double diffusion (AGDD) and a standard ELISA procedure for detecting antibodies to Aspergillus fumigatus in 28 asthmatic patients with suspected allergic aspergillosis. Using two A fumigatus antigens the rapid ELISA compared well with AGDD and the standard ELISA method. Eleven sera that reacted with both antigens in AGDD were all positive against antigen 1 in both forms of ELISA, but two failed to react with antigen 2 in the standard ELISA and three failed to react with this antigen in the rapid method. Thirteen AGDD-negative sera were also negative in both forms of ELISA. The rapid ELISA provides a sensitive and reproducible test for routine serological investigation of allergic aspergillosis.
    Original languageEnglish
    Pages (from-to)1134-1137
    Number of pages3
    JournalJournal of Clinical Pathology
    Issue number10
    Publication statusPublished - 1982


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