Rapid prototyping of microbial production strains for the biomanufacture of potential material monomers

Christopher Robinson; Pablo Carbonell; Adrian Jervis; Cunyu Yan; Katherine Hollywood; Andrew Currin; Neil Swainston; Reynard Speiss; Sandra Taylor; Paul Mulherin; Steven Parker; William Rowe; Nick Matthews; Kirk Malone; Rosalind Le Feuvre; Philip Shapira; Perdita Barran; Nicholas Turner; Jason Micklefield; Rainer Breitling; Eriko Takano; Nigel Scrutton

doi:10.1016/j.ymben.2020.04.008

Rapid prototyping of microbial production strains for the biomanufacture of potential material monomers

Christopher Robinson, Pablo Carbonell, Adrian Jervis, Cunyu Yan, Katherine Hollywood, Andrew Currin, Neil Swainston, Reynard Speiss, Sandra Taylor, Paul Mulherin, Steven Parker, William Rowe, Nick Matthews, Kirk Malone, Rosalind Le Feuvre, Philip Shapira, Perdita Barran, Nicholas Turner, Jason Micklefield, Rainer BreitlingEriko Takano, Nigel Scrutton

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Abstract

Bio-based production of industrial chemicals using synthetic biology can provide alternative green routes from renewable resources, allowing for cleaner production processes. To efficiently produce chemicals on-demand through microbial strain engineering, biomanufacturing foundries have developed automated pipelines that are largely compound agnostic in their time to delivery. Here we benchmark the capabilities of a biomanufacturing pipeline to enable rapid prototyping of microbial cell factories for the production of chemically diverse industrially relevant material building blocks. Over 85 days the pipeline was able to produce 17 potential material monomers and key intermediates
by combining 160 genetic parts into 115 unique biosynthetic pathways. To explore the scale-up potential of our prototype production strains, we optimized the enantioselective production of mandelic acid and hydroxymandelic acid, achieving gram-scale production in fed-batch fermenters. The high success rate in the rapid design and prototyping of microbially-produced material building
blocks reveals the potential role of biofoundries in leading the transition to sustainable materials
production.

Original language	English
Journal	Metabolic Engineering
Early online date	23 Apr 2020
DOIs	https://doi.org/10.1016/j.ymben.2020.04.008
Publication status	Published - 1 Jul 2020

Keywords

synthetic biology
biofoundry
biomanufacturing
industrial biotechnology
mandelic acid
hydroxymandelic acid

Research Beacons, Institutes and Platforms

Manchester Institute of Biotechnology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.ymben.2020.04.008Licence: CC BY

1-s2.0-S109671762030077X-mainFinal published version, 8.29 MBLicence: CC BY

Cite this

Robinson, C., Carbonell, P., Jervis, A., Yan, C., Hollywood, K., Currin, A., Swainston, N., Speiss, R., Taylor, S., Mulherin, P., Parker, S., Rowe, W., Matthews, N., Malone, K., Le Feuvre, R., Shapira, P., Barran, P., Turner, N., Micklefield, J., ... Scrutton, N. (2020). Rapid prototyping of microbial production strains for the biomanufacture of potential material monomers. Metabolic Engineering. https://doi.org/10.1016/j.ymben.2020.04.008

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abstract = "Bio-based production of industrial chemicals using synthetic biology can provide alternative green routes from renewable resources, allowing for cleaner production processes. To efficiently produce chemicals on-demand through microbial strain engineering, biomanufacturing foundries have developed automated pipelines that are largely compound agnostic in their time to delivery. Here we benchmark the capabilities of a biomanufacturing pipeline to enable rapid prototyping of microbial cell factories for the production of chemically diverse industrially relevant material building blocks. Over 85 days the pipeline was able to produce 17 potential material monomers and key intermediatesby combining 160 genetic parts into 115 unique biosynthetic pathways. To explore the scale-up potential of our prototype production strains, we optimized the enantioselective production of mandelic acid and hydroxymandelic acid, achieving gram-scale production in fed-batch fermenters. The high success rate in the rapid design and prototyping of microbially-produced material buildingblocks reveals the potential role of biofoundries in leading the transition to sustainable materialsproduction.",

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Robinson, C, Carbonell, P, Jervis, A, Yan, C, Hollywood, K, Currin, A, Swainston, N, Speiss, R, Taylor, S, Mulherin, P, Parker, S, Rowe, W, Matthews, N, Malone, K, Le Feuvre, R , Shapira, P , Barran, P , Turner, N , Micklefield, J , Breitling, R , Takano, E & Scrutton, N 2020, 'Rapid prototyping of microbial production strains for the biomanufacture of potential material monomers', Metabolic Engineering. https://doi.org/10.1016/j.ymben.2020.04.008

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AU - Swainston, Neil

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AU - Turner, Nicholas

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AU - Breitling, Rainer

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Rapid prototyping of microbial production strains for the biomanufacture of potential material monomers

Abstract

Keywords

Research Beacons, Institutes and Platforms

UN SDGs

Access to Document

Other files and links

Fingerprint

Manchester Synthetic Biology Research Centre for Fine and Speciality Chemicals

Biochemical and Biophysical Sciences Technology Platform

Cite this

Rapid prototyping of microbial production strains for the biomanufacture of potential material monomers

Abstract

Keywords

Research Beacons, Institutes and Platforms

UN SDGs

Access to Document

Other files and links

Fingerprint

Projects

Manchester Synthetic Biology Research Centre for Fine and Speciality Chemicals

Equipment

Biochemical and Biophysical Sciences Technology Platform

Cite this