Rapid stimulation of cyclic AMP production by aldosterone in rat inner medullary collecting ducts

E. A. Sheader, E. T. Wargent, N. Ashton, R. J. Balment

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Aldosterone stimulates sodium transport in the inner medullary collecting duct (IMCD) via the classic genomic pathway, but it is not known whether it also acts via a rapid, non-conventional pathway in this part of the nephron. The IMCD regulates the final sodium content of urine and expresses vasopressin receptors coupled to adenylate cyclase. The recently reported rapid, nongenomic actions of aldosterone have been associated mainly with an increase in intracellular Ca2+ ; however, it has also been shown to stimulate cAMP generation. Thus the aim of this study was to determine whether aldosterone stimulates rapid generation of cAMP in isolated IMCD segments. IMCD segments were microdissected from Sprague-Dawley rat kidneys and incubated at 37 °C for 4 min with aldosterone (10-12 to 10-6 M), vasopressin (10-12 to 10-6 M), or a combination of hormones in the presence of a phosphodiesterase inhibitor. cAMP was measured by radioimmunoassay. While corticosterone and dexamethasone were ineffective, aldosterone stimulated a dose-dependent increase in cAMP within 4 min (P
    Original languageEnglish
    Pages (from-to)343-347
    Number of pages4
    JournalJournal of Endocrinology
    Volume175
    Issue number2
    DOIs
    Publication statusPublished - 1 Nov 2002

    Fingerprint

    Dive into the research topics of 'Rapid stimulation of cyclic AMP production by aldosterone in rat inner medullary collecting ducts'. Together they form a unique fingerprint.

    Cite this