RAS at the Golgi antagonizes malignant transformation through PTPRκ-mediated inhibition of ERK activation

Berta Casar, Andrew P Badrock, Iñaki Jiménez, Imanol Arozarena, Paula Colón-Bolea, L Francisco Lorenzo-Martín, Irene Barinaga-Rementería, Jorge Barriuso, Vincenzo Cappitelli, Daniel J Donoghue, Xosé R Bustelo, Adam Hurlstone, Piero Crespo

Research output: Contribution to journalArticlepeer-review

Abstract

RAS GTPases are frequently mutated in human cancer. H- and NRAS isoforms are distributed over both plasma-membrane and endomembranes, including the Golgi complex, but how this organizational context contributes to cellular transformation is unknown. Here we show that RAS at the Golgi is selectively activated by apoptogenic stimuli and antagonizes cell survival by suppressing ERK activity through the induction of PTPRκ, which targets CRAF for dephosphorylation. Consistently, in contrast to what occurs at the plasma-membrane, RAS at the Golgi cannot induce melanoma in zebrafish. Inactivation of PTPRκ, which occurs frequently in human melanoma, often coincident with TP53 inactivation, accelerates RAS-ERK pathway-driven melanomagenesis in zebrafish. Likewise, tp53 disruption in zebrafish facilitates oncogenesis driven by RAS from the Golgi complex. Thus, RAS oncogenic potential is strictly dependent on its sublocalization, with Golgi complex-located RAS antagonizing tumor development.

Original languageEnglish
Article number3595
Pages (from-to)3595
JournalNature Communications
Volume9
Issue number1
Early online date5 Sept 2018
DOIs
Publication statusPublished - 2018

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