Ratiometric detection of enzyme turnover and flavin reduction using rare-earth upconverting phosphors

Peter Harvey; Chloë Oakland; Max D. Driscoll; Sam Hay; Louise S. Natrajan

doi:10.1039/c4dt00356j

Ratiometric detection of enzyme turnover and flavin reduction using rare-earth upconverting phosphors

Peter Harvey, Chloë Oakland, Max D. Driscoll, Sam Hay, Louise S. Natrajan

Research output: Contribution to journal › Article › peer-review

Abstract

Gd4O2S:Yb:Tm rare-earth upconversion phosphors have been utilised to monitor the redox behaviour of flavin mononucleotide and report on the turnover of a flavo-protein, (pentaerythritol tetranitrate reductase). The presence of two bands separated by over 300 nm in the UCP emission spectra allows ratiometric signalling of these processes with high sensitivity. This journal is © the Partner Organisations 2014.

Original language	English
Pages (from-to)	5265-5268
Number of pages	3
Journal	Dalton Transactions
Volume	43
Issue number	14
DOIs	https://doi.org/10.1039/c4dt00356j
Publication status	Published - 14 Apr 2014

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10.1039/c4dt00356j

Linking experiment to theory: Quantum entanglement during enzyme catalysis - Dr S Hay fellowship
Hay, S. (PI)
1/09/10 → 31/08/15
Project: Research

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title = "Ratiometric detection of enzyme turnover and flavin reduction using rare-earth upconverting phosphors",

abstract = "Gd4O2S:Yb:Tm rare-earth upconversion phosphors have been utilised to monitor the redox behaviour of flavin mononucleotide and report on the turnover of a flavo-protein, (pentaerythritol tetranitrate reductase). The presence of two bands separated by over 300 nm in the UCP emission spectra allows ratiometric signalling of these processes with high sensitivity. This journal is {\textcopyright} the Partner Organisations 2014.",

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AU - Oakland, Chloë

AU - Driscoll, Max D.

AU - Hay, Sam

AU - Natrajan, Louise S.

PY - 2014/4/14

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AB - Gd4O2S:Yb:Tm rare-earth upconversion phosphors have been utilised to monitor the redox behaviour of flavin mononucleotide and report on the turnover of a flavo-protein, (pentaerythritol tetranitrate reductase). The presence of two bands separated by over 300 nm in the UCP emission spectra allows ratiometric signalling of these processes with high sensitivity. This journal is © the Partner Organisations 2014.

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DO - 10.1039/c4dt00356j

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SP - 5265

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JO - Dalton Transactions

JF - Dalton Transactions

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Ratiometric detection of enzyme turnover and flavin reduction using rare-earth upconverting phosphors

Abstract

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Projects

Linking experiment to theory: Quantum entanglement during enzyme catalysis - Dr S Hay fellowship

Cite this