Rational design of a cyclin A fluorescent peptide sensor

Elena Pazos, Miguel Pérez, Hugo Gutiérrez-de-Terán, Mar Orzáez, Centro de Investigación Felipe, José L Mascareñas, M Eugenio Vázquez

Research output: Contribution to journalArticlepeer-review


We report the design and development of a fluorescent sensor specifically designed to target cyclin A, a protein that plays a key role in the regulation of the cell cycle. Computational studies provide a molecular picture that explains the observed emission increase, suggesting that the 4-DMAP fluorophore in the peptide is protected from the bulk solvent when inserted into the hydrophobic binding groove of cyclin A.

Original languageEnglish
Pages (from-to)7629-32
Number of pages4
JournalOrganic & biomolecular chemistry
Issue number22
Publication statusPublished - 26 Oct 2011
Externally publishedYes


  • Binding Sites
  • Biological Assay
  • Biosensing Techniques/methods
  • Cell Cycle/drug effects
  • Cell Proliferation/drug effects
  • Cyclin A/antagonists & inhibitors
  • Cyclin-Dependent Kinases/antagonists & inhibitors
  • Fluorescent Dyes/chemistry
  • HeLa Cells
  • Humans
  • Models, Molecular
  • Molecular Imprinting/methods
  • Neoplasms/drug therapy
  • Peptide Library
  • Peptides/chemical synthesis
  • Phthalimides/chemistry
  • Protein Binding
  • Protein Kinase Inhibitors/metabolism
  • Spectrometry, Fluorescence
  • Staining and Labeling/methods

Research Beacons, Institutes and Platforms

  • Manchester Cancer Research Centre


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