Rational design of a cyclin A fluorescent peptide sensor

Elena Pazos; Miguel Pérez; Hugo Gutiérrez-de-Terán; Mar Orzáez; Centro de Investigación Felipe; José L Mascareñas; M Eugenio Vázquez

doi:10.1039/c1ob06009k

Rational design of a cyclin A fluorescent peptide sensor

Elena Pazos, Miguel Pérez, Hugo Gutiérrez-de-Terán, Mar Orzáez, Centro de Investigación Felipe, José L Mascareñas, M Eugenio Vázquez

Research output: Contribution to journal › Article › peer-review

Abstract

We report the design and development of a fluorescent sensor specifically designed to target cyclin A, a protein that plays a key role in the regulation of the cell cycle. Computational studies provide a molecular picture that explains the observed emission increase, suggesting that the 4-DMAP fluorophore in the peptide is protected from the bulk solvent when inserted into the hydrophobic binding groove of cyclin A.

Original language	English
Pages (from-to)	7629-32
Number of pages	4
Journal	Organic & biomolecular chemistry
Volume	9
Issue number	22
DOIs	https://doi.org/10.1039/c1ob06009k
Publication status	Published - 26 Oct 2011
Externally published	Yes

Keywords

Binding Sites
Biological Assay
Biosensing Techniques/methods
Cell Cycle/drug effects
Cell Proliferation/drug effects
Cyclin A/antagonists & inhibitors
Cyclin-Dependent Kinases/antagonists & inhibitors
Fluorescent Dyes/chemistry
HeLa Cells
Humans
Models, Molecular
Molecular Imprinting/methods
Neoplasms/drug therapy
Peptide Library
Peptides/chemical synthesis
Phthalimides/chemistry
Protein Binding
Protein Kinase Inhibitors/metabolism
Spectrometry, Fluorescence
Staining and Labeling/methods

Research Beacons, Institutes and Platforms

Manchester Cancer Research Centre

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1039/c1ob06009k

Cite this

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title = "Rational design of a cyclin A fluorescent peptide sensor",

abstract = "We report the design and development of a fluorescent sensor specifically designed to target cyclin A, a protein that plays a key role in the regulation of the cell cycle. Computational studies provide a molecular picture that explains the observed emission increase, suggesting that the 4-DMAP fluorophore in the peptide is protected from the bulk solvent when inserted into the hydrophobic binding groove of cyclin A.",

keywords = "Binding Sites, Biological Assay, Biosensing Techniques/methods, Cell Cycle/drug effects, Cell Proliferation/drug effects, Cyclin A/antagonists & inhibitors, Cyclin-Dependent Kinases/antagonists & inhibitors, Fluorescent Dyes/chemistry, HeLa Cells, Humans, Models, Molecular, Molecular Imprinting/methods, Neoplasms/drug therapy, Peptide Library, Peptides/chemical synthesis, Phthalimides/chemistry, Protein Binding, Protein Kinase Inhibitors/metabolism, Spectrometry, Fluorescence, Staining and Labeling/methods",

author = "Elena Pazos and Miguel P{\'e}rez and Hugo Guti{\'e}rrez-de-Ter{\'a}n and Mar Orz{\'a}ez and Felipe, {Centro de Investigaci{\'o}n} and Mascare{\~n}as, {Jos{\'e} L} and V{\'a}zquez, {M Eugenio}",

year = "2011",

month = oct,

day = "26",

doi = "10.1039/c1ob06009k",

language = "English",

volume = "9",

pages = "7629--32",

journal = "Organic & biomolecular chemistry",

issn = "1477-0520",

publisher = "Royal Society of Chemistry",

number = "22",

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TY - JOUR

T1 - Rational design of a cyclin A fluorescent peptide sensor

AU - Pazos, Elena

AU - Pérez, Miguel

AU - Gutiérrez-de-Terán, Hugo

AU - Orzáez, Mar

AU - Felipe, Centro de Investigación

AU - Mascareñas, José L

AU - Vázquez, M Eugenio

PY - 2011/10/26

Y1 - 2011/10/26

N2 - We report the design and development of a fluorescent sensor specifically designed to target cyclin A, a protein that plays a key role in the regulation of the cell cycle. Computational studies provide a molecular picture that explains the observed emission increase, suggesting that the 4-DMAP fluorophore in the peptide is protected from the bulk solvent when inserted into the hydrophobic binding groove of cyclin A.

AB - We report the design and development of a fluorescent sensor specifically designed to target cyclin A, a protein that plays a key role in the regulation of the cell cycle. Computational studies provide a molecular picture that explains the observed emission increase, suggesting that the 4-DMAP fluorophore in the peptide is protected from the bulk solvent when inserted into the hydrophobic binding groove of cyclin A.

KW - Binding Sites

KW - Biological Assay

KW - Biosensing Techniques/methods

KW - Cell Cycle/drug effects

KW - Cell Proliferation/drug effects

KW - Cyclin A/antagonists & inhibitors

KW - Cyclin-Dependent Kinases/antagonists & inhibitors

KW - Fluorescent Dyes/chemistry

KW - HeLa Cells

KW - Humans

KW - Models, Molecular

KW - Molecular Imprinting/methods

KW - Neoplasms/drug therapy

KW - Peptide Library

KW - Peptides/chemical synthesis

KW - Phthalimides/chemistry

KW - Protein Binding

KW - Protein Kinase Inhibitors/metabolism

KW - Spectrometry, Fluorescence

KW - Staining and Labeling/methods

U2 - 10.1039/c1ob06009k

DO - 10.1039/c1ob06009k

M3 - Article

C2 - 21863156

SN - 1477-0520

VL - 9

SP - 7629

EP - 7632

JO - Organic & biomolecular chemistry

JF - Organic & biomolecular chemistry

IS - 22

ER -

Rational design of a cyclin A fluorescent peptide sensor

Abstract

Keywords

Research Beacons, Institutes and Platforms

UN SDGs

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