Real-time biomolecular interaction analysis using the resonant mirror sensor

Nicholas J. Goddard, Denise Pollard-Knight, Colin H. Maule

    Research output: Contribution to journalArticlepeer-review

    Abstract

    The resonant mirror is a planar waveguide optical sensor that uses frustrated total internal reflection to couple light in and out of the waveguide layer. As a biosensor, the device exploits the highly selective binding interactions between pairs of biomolecules such as enzyme-substrate, antibody-antigen, hormone-receptor and DNA-DNA (DNA = deoxyribonucleic acid). As many of these species have no absorption bands at convenient (visible) wavelengths, refractive index sensing is employed to detect the displacement of water by the higher refractive index analyte as it interacts with its immobilized binding partner. The sensor chip construction is relatively simple, using techniques developed for the production of anti-reflection coatings and interference filters. Preliminary experimental results are presented from two instrumental configurations using a single type of sensor chip. Both types of instrument use linear or two-dimensional charge coupled device (CCD) arrays as detectors, thus avoiding the use of moving parts in the instrumentation.
    Original languageEnglish
    Pages (from-to)583-588
    Number of pages5
    JournalAnalyst
    Volume119
    Issue number4
    DOIs
    Publication statusPublished - 1994

    Keywords

    • Immunoassay (immunosensors based on resonant mirror sensors for)
    • Biosensors (resonant mirror-based, biomol. interaction anal. by)
    • Molecules (biochem., interactions of, anal. of, resonant mirror sensors for)
    • Biosensors (immunol., resonant mirror-based, biomol. interaction anal. by)
    • Waveguides (optical, planar, frustrated total internal reflection in, in biosensors)
    • resonant mirror biosensor biomol interaction analysis
    • immunosensor resonant mirror waveguide optical sensor

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