Real-time screening of biocatalysts in live bacterial colonies

Cunyu Yan; Fabio Parmeggiani; Emrys A. Jones; Emmanuelle Claude; Shaneela Hussain; Nicholas Turner; Sabine Flitsch; Perdita Barran

doi:10.1021/jacs.6b12165

Real-time screening of biocatalysts in live bacterial colonies

Cunyu Yan, Fabio Parmeggiani, Emrys A. Jones, Emmanuelle Claude, Shaneela Hussain, Nicholas Turner, Sabine Flitsch, Perdita Barran

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Abstract

Screening of bacterial colonies to identify new biocatalytic activities is a widely adopted tool in biotechnology, but is constrained by the requirements for colorimetric or tag-based detection methods. Herein we report a label-free screening platform using desorption electrospray ionization coupled with ion mobility mass spectrometry imaging (DESI-IM-MS). The screening method is demonstrated for both ammonia lyases and P450 monooxygenases expressed within live bacterial colonies and is shown to enable multiplexing of enzyme variants and substrate libraries simultaneously.

Original language	English
Pages (from-to)	1408-1411
Number of pages	4
Journal	Journal of the American Chemical Society
Volume	139
Issue number	4
Early online date	13 Jan 2017
DOIs	https://doi.org/10.1021/jacs.6b12165
Publication status	Published - 2017

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Manchester Institute of Biotechnology

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10.1021/jacs.6b12165

DiBT screening manuscript resubAccepted author manuscript, 2.01 MB

Manchester Synthetic Biology Research Centre for Fine and Speciality Chemicals
Scrutton, N. (PI), Azapagic, A. (CoI), Balmer, A. (CoI), Barran, P. (CoI), Breitling, R. (CoI), Delneri, D. (CoI), Dixon, N. (CoI), Faulon, J.-L. (CoI), Flitsch, S. (CoI), Goble, C. (CoI), Goodacre, R. (CoI), Hay, S. (CoI), Kell, D. (CoI), Leys, D. (CoI), Lloyd, J. (CoI), Lockyer, N. (CoI), Martin, P. (CoI), Micklefield, J. (CoI), Munro, A. (CoI), Pedrosa Mendes, P. (CoI), Randles, S. (CoI), Salehi Yazdi, F. (CoI), Shapira, P. (CoI), Takano, E. (CoI), Turner, N. (CoI) & Winterburn, J. (CoI)
14/11/14 → 13/05/20
Project: Research

Cite this

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title = "Real-time screening of biocatalysts in live bacterial colonies",

abstract = "Screening of bacterial colonies to identify new biocatalytic activities is a widely adopted tool in biotechnology, but is constrained by the requirements for colorimetric or tag-based detection methods. Herein we report a label-free screening platform using desorption electrospray ionization coupled with ion mobility mass spectrometry imaging (DESI-IM-MS). The screening method is demonstrated for both ammonia lyases and P450 monooxygenases expressed within live bacterial colonies and is shown to enable multiplexing of enzyme variants and substrate libraries simultaneously.",

author = "Cunyu Yan and Fabio Parmeggiani and Jones, {Emrys A.} and Emmanuelle Claude and Shaneela Hussain and Nicholas Turner and Sabine Flitsch and Perdita Barran",

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T1 - Real-time screening of biocatalysts in live bacterial colonies

AU - Yan, Cunyu

AU - Parmeggiani, Fabio

AU - Jones, Emrys A.

AU - Claude, Emmanuelle

AU - Hussain, Shaneela

AU - Turner, Nicholas

AU - Flitsch, Sabine

AU - Barran, Perdita

PY - 2017

Y1 - 2017

N2 - Screening of bacterial colonies to identify new biocatalytic activities is a widely adopted tool in biotechnology, but is constrained by the requirements for colorimetric or tag-based detection methods. Herein we report a label-free screening platform using desorption electrospray ionization coupled with ion mobility mass spectrometry imaging (DESI-IM-MS). The screening method is demonstrated for both ammonia lyases and P450 monooxygenases expressed within live bacterial colonies and is shown to enable multiplexing of enzyme variants and substrate libraries simultaneously.

AB - Screening of bacterial colonies to identify new biocatalytic activities is a widely adopted tool in biotechnology, but is constrained by the requirements for colorimetric or tag-based detection methods. Herein we report a label-free screening platform using desorption electrospray ionization coupled with ion mobility mass spectrometry imaging (DESI-IM-MS). The screening method is demonstrated for both ammonia lyases and P450 monooxygenases expressed within live bacterial colonies and is shown to enable multiplexing of enzyme variants and substrate libraries simultaneously.

U2 - 10.1021/jacs.6b12165

DO - 10.1021/jacs.6b12165

M3 - Article

SN - 0002-7863

VL - 139

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JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

IS - 4

ER -

Real-time screening of biocatalysts in live bacterial colonies

Abstract

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