Rearrangement and expression of immunoglobulin light chain genes can precede heavy chain expression during normal B cell development in mice

Tatiana I. Novobrantseva, Verena M. Martin, Roberta Pelanda, Werner Müller, Klaus Rajewsky, Andreas Ehlich

    Research output: Contribution to journalArticlepeer-review

    Abstract

    In mouse mutants incapable of expressing μ chains, V(K)J(K)joints are detected in the CD43+ B cell progenitors. In agreement with these earlier results, we show by a molecular single cell analysis that 4-7% of CD43+ B cell progenitors in wild-type mice rearrange immunoglobulin (Ig)κ genes before the assembly of a productive V(H)D(H)J(H) joint. Thus, μ chain expression is not a prerequisite to Igκ light chain gene rearrangements in normal development. Overall, ~15% of the total CD43+ B cell progenitor population carry Igκ gene rearrangements in wild-type mice. Together with the results obtained in the mouse mutants, these data fit a model in which CD43+ progenitors rearrange IgH and Igκ loci independently, with a seven times higher frequency in the former. In addition, we show that in B cell progenitors V(K)J(K)joining rapidly initiates κ chain expression, irrespective of the presence of a μ chain.
    Original languageEnglish
    Pages (from-to)75-87
    Number of pages12
    JournalJournal of Experimental Medicine
    Volume189
    Issue number1
    DOIs
    Publication statusPublished - 4 Jan 1999

    Keywords

    • B cell development
    • Bone marrow
    • Immunoglobulin gene rearrangement

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