Receiver operating characteristic curve analysis of four Aspergillus-specific IgG assays for the diagnosis of chronic pulmonary aspergillosis

Iain Page, Caroline Baxter, Christophe Hennequin, Malcolm Richardson, Erna van Hoeyveld, Albert van Toorenenbergen, David Denning

Research output: Contribution to journalArticlepeer-review

78 Downloads (Pure)

Abstract

Measurement of Aspergillus-specific IgG is central to the diagnosis of chronic pulmonary aspergillosis (CPA). We calculated diagnostic cut-offs for this immunoglobulin.

Aspergillus-specific IgG levels were measured in sera from adults with CPA and healthy controls by ImmunoCAP, Immulite, Serion and Bio-Rad assays. Receiver operating characteristic (ROC) area under the curve (AUC) analysis was performed to identify optimal cut-offs.

ROC AUC results were; Bio-Rad 0.955, Immulite 0.948, ImmunoCAP 0.956, and Serion 0.944. Optimal diagnostic cut-offs were 1.5 AU/ml for Bio-Rad (93% sensitive, 98% specific), 25 mg/L for Immulite (93% sensitive, 99% specific), 50 mg/L for ImmunoCAP (84% sensitive, 96% specific) and 50 U/ml for Serion (84% sensitive, 91% specific).

This is the first ROC AUC analysis of Aspergillus-specific IgG in CPA patients using European controls. Cut-offs identified using European controls are likely to be more accurate in a developed, temperate setting than the cut-offs we previously identified using African controls.
Original languageEnglish
JournalDiagnostic Microbiology and Infectious Disease
Early online date6 Jan 2018
DOIs
Publication statusPublished - 2018

Fingerprint

Dive into the research topics of 'Receiver operating characteristic curve analysis of four Aspergillus-specific IgG assays for the diagnosis of chronic pulmonary aspergillosis'. Together they form a unique fingerprint.

Cite this