Reconstruction of the Global Neural Crest Gene Regulatory Network In Vivo

Ruth M Williams, Ivan Candido-Ferreira, Emmanouela Repapi, Daria Gavriouchkina, Upeka Senanayake, Irving T C Ling, Jelena Telenius, Stephen Taylor, Jim Hughes, Tatjana Sauka-Spengler

Research output: Contribution to journalArticlepeer-review

Abstract

Precise control of developmental processes is encoded in the genome in the form of gene regulatory networks (GRNs). Such multi-factorial systems are difficult to decode in vertebrates owing to their complex gene hierarchies and dynamic molecular interactions. Here we present a genome-wide in vivo reconstruction of the GRN underlying development of the multipotent neural crest (NC) embryonic cell population. By coupling NC-specific epigenomic and transcriptional profiling at population and single-cell levels with genome/epigenome engineering in vivo, we identify multiple regulatory layers governing NC ontogeny, including NC-specific enhancers and super-enhancers, novel trans-factors, and cis-signatures allowing reverse engineering of the NC-GRN at unprecedented resolution. Furthermore, identification and dissection of divergent upstream combinatorial regulatory codes has afforded new insights into opposing gene circuits that define canonical and neural NC fates early during NC ontogeny. Our integrated approach, allowing dissection of cell-type-specific regulatory circuits in vivo, has broad implications for GRN discovery and investigation.

Original languageEnglish
Pages (from-to)255-276.e7
JournalDevelopmental cell
Volume51
Issue number2
DOIs
Publication statusPublished - 21 Oct 2019

Keywords

  • Animals
  • Gene Expression Regulation, Developmental/genetics
  • Gene Regulatory Networks/genetics
  • Genetic Heterogeneity
  • Neural Crest/embryology
  • Transcriptional Activation/genetics
  • Vertebrates/genetics

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