Redundant control of migration and adhesion by ERM proteins in vascular smooth muscle cells

Nicolas Baeyens, Iman Latrache, Xavier Yerna, Gauthier Noppe, Sandrine Horman, Nicole Morel

    Research output: Contribution to journalArticlepeer-review


    Ezrin, radixin, and moesin possess a very similar structure with a C-terminal actin-binding domain and a N-terminal FERM interacting domain. They are known to be involved in cytoskeleton organization in several cell types but their function in vascular smooth muscle cells (VSMC) is still unknown. The aim of this study was to investigate the role of ERM proteins in cell migration induced by PDGF, a growth factor involved in pathophysiological processes like angiogenesis or atherosclerosis. We used primary cultured VSMC obtained from rat aorta, which express the three ERM proteins. Simultaneous depletion of the three ERM proteins with specific siRNAs abolished the effects of PDGF on cell architecture and migration and markedly increased cell adhesion and focal adhesion size, while these parameters were only slightly affected by depletion of ezrin, radixin or moesin alone. Rac1 activation, cell proliferation, and Ca(2+) signal in response to PDGF were unaffected by ERM depletion. These results indicate that ERM proteins exert a redundant control on PDGF-induced VSMC migration by regulating focal adhesion turn-over and cell adhesion to substrate.

    Original languageEnglish
    Pages (from-to)579-85
    Number of pages7
    JournalBiochemical and biophysical research communications
    Issue number3
    Publication statusPublished - 22 Nov 2013


    • Animals
    • Cell Adhesion
    • Cell Movement
    • Cells, Cultured
    • Cytoskeletal Proteins
    • Focal Adhesions
    • Male
    • Membrane Proteins
    • Microfilament Proteins
    • Muscle, Smooth, Vascular
    • Myocytes, Smooth Muscle
    • Platelet-Derived Growth Factor
    • Pseudopodia
    • Rats
    • Rats, Wistar


    Dive into the research topics of 'Redundant control of migration and adhesion by ERM proteins in vascular smooth muscle cells'. Together they form a unique fingerprint.

    Cite this