Regulated toluene cis-glycol production by recombinant Escherichia coli strains constructed by PCR amplification of the toluene dioxygenase genes from Pseudomonas putida

L. P. Wahbi, P. Phumathon, A. Brown, S. Minter, G. M. Stephens

    Research output: Contribution to journalArticlepeer-review

    Abstract

    The toluene dioxygenase genes from Pseudomonas pufida NCIMB 11767 were isolated by PCR amplification from recombinant plasmid, p1/1. The genes were subcloned into pUC18 and pKK223-3 and expressed under the lac and tac promoters, respectively. In both cases, toluene cis-glycol was produced, with higher levels of product formation when the genes were expressed from the tac promoter.
    Original languageEnglish
    Pages (from-to)961-965
    Number of pages4
    JournalBiotechnology Letters
    Volume19
    Issue number10
    DOIs
    Publication statusPublished - 1997

    Keywords

    • Escherichia coli
    • Molecular cloning
    • Pseudomonas putida (regulated toluene cis-glycol prodn. by recombinant Escherichia coli strains constructed by PCR amplification of toluene dioxygenase genes from Pseudomonas putida)
    • Gene Role: BPR (Biological process), BSU (Biological study, unclassified), BIOL (Biological study), PROC (Process) (regulated toluene cis-glycol prodn. by recombinant Escherichia coli strains constructed by PCR amplification of toluene dioxygenase genes
    • toluene glycol prodn recombinant Escherichia dioxygenase
    • Pseudomonas toluene dioxygenase gene cloning Escherichia

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