Abstract
The toluene dioxygenase genes from Pseudomonas pufida NCIMB 11767 were isolated by PCR amplification from recombinant plasmid, p1/1. The genes were subcloned into pUC18 and pKK223-3 and expressed under the lac and tac promoters, respectively. In both cases, toluene cis-glycol was produced, with higher levels of product formation when the genes were expressed from the tac promoter.
Original language | English |
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Pages (from-to) | 961-965 |
Number of pages | 4 |
Journal | Biotechnology Letters |
Volume | 19 |
Issue number | 10 |
DOIs | |
Publication status | Published - 1997 |
Keywords
- Escherichia coli
- Molecular cloning
- Pseudomonas putida (regulated toluene cis-glycol prodn. by recombinant Escherichia coli strains constructed by PCR amplification of toluene dioxygenase genes from Pseudomonas putida)
- Gene Role: BPR (Biological process), BSU (Biological study, unclassified), BIOL (Biological study), PROC (Process) (regulated toluene cis-glycol prodn. by recombinant Escherichia coli strains constructed by PCR amplification of toluene dioxygenase genes
- toluene glycol prodn recombinant Escherichia dioxygenase
- Pseudomonas toluene dioxygenase gene cloning Escherichia