Regulated toluene cis-glycol production by recombinant Escherichia coli strains constructed by PCR amplification of the toluene dioxygenase genes from Pseudomonas putida

L. P. Wahbi; P. Phumathon; A. Brown; S. Minter; G. M. Stephens

doi:10.1023/A:1018492630150

Regulated toluene cis-glycol production by recombinant Escherichia coli strains constructed by PCR amplification of the toluene dioxygenase genes from Pseudomonas putida

L. P. Wahbi
, P. Phumathon
, A. Brown
, S. Minter
, G. M. Stephens

Research output: Contribution to journal › Article › peer-review

Abstract

The toluene dioxygenase genes from Pseudomonas pufida NCIMB 11767 were isolated by PCR amplification from recombinant plasmid, p1/1. The genes were subcloned into pUC18 and pKK223-3 and expressed under the lac and tac promoters, respectively. In both cases, toluene cis-glycol was produced, with higher levels of product formation when the genes were expressed from the tac promoter.

Original language	English
Pages (from-to)	961-965
Number of pages	4
Journal	Biotechnology Letters
Volume	19
Issue number	10
DOIs	https://doi.org/10.1023/A:1018492630150
Publication status	Published - 1997

Keywords

Escherichia coli
Molecular cloning
Pseudomonas putida (regulated toluene cis-glycol prodn. by recombinant Escherichia coli strains constructed by PCR amplification of toluene dioxygenase genes from Pseudomonas putida)
Gene Role: BPR (Biological process), BSU (Biological study, unclassified), BIOL (Biological study), PROC (Process) (regulated toluene cis-glycol prodn. by recombinant Escherichia coli strains constructed by PCR amplification of toluene dioxygenase genes
toluene glycol prodn recombinant Escherichia dioxygenase
Pseudomonas toluene dioxygenase gene cloning Escherichia

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title = "Regulated toluene cis-glycol production by recombinant Escherichia coli strains constructed by PCR amplification of the toluene dioxygenase genes from Pseudomonas putida",

abstract = "The toluene dioxygenase genes from Pseudomonas pufida NCIMB 11767 were isolated by PCR amplification from recombinant plasmid, p1/1. The genes were subcloned into pUC18 and pKK223-3 and expressed under the lac and tac promoters, respectively. In both cases, toluene cis-glycol was produced, with higher levels of product formation when the genes were expressed from the tac promoter.",

keywords = "Escherichia coli, Molecular cloning, Pseudomonas putida (regulated toluene cis-glycol prodn. by recombinant Escherichia coli strains constructed by PCR amplification of toluene dioxygenase genes from Pseudomonas putida), Gene Role: BPR (Biological process), BSU (Biological study, unclassified), BIOL (Biological study), PROC (Process) (regulated toluene cis-glycol prodn. by recombinant Escherichia coli strains constructed by PCR amplification of toluene dioxygenase genes, toluene glycol prodn recombinant Escherichia dioxygenase, Pseudomonas toluene dioxygenase gene cloning Escherichia",

author = "Wahbi, \{L. P.\} and P. Phumathon and A. Brown and S. Minter and Stephens, \{G. M.\}",

note = "CAN 128:12604 16-5 Fermentation and Bioindustrial Chemistry Department of Chemical Engineering, UMIST,Manchester,UK. Journal 0141-5492 written in English. 120038-36-0 (Toluene dioxygenase) Role: BAC (Biological activity or effector, except adverse), BSU (Biological study, unclassified), BIOL (Biological study) (regulated toluene cis-glycol prodn. by recombinant Escherichia coli strains constructed by PCR amplification of toluene dioxygenase genes from Pseudomonas putida); 41977-20-2P (Toluene cis-glycol) Role: BMF (Bioindustrial manufacture), BIOL (Biological study), PREP (Preparation) (regulated toluene cis-glycol prodn. by recombinant Escherichia coli strains constructed by PCR amplification of toluene dioxygenase genes from Pseudomonas putida)",

year = "1997",

doi = "10.1023/A:1018492630150",

language = "English",

volume = "19",

pages = "961--965",

journal = "Biotechnology Letters",

issn = "0141-5492",

publisher = "Springer Nature",

number = "10",

}

TY - JOUR

T1 - Regulated toluene cis-glycol production by recombinant Escherichia coli strains constructed by PCR amplification of the toluene dioxygenase genes from Pseudomonas putida

AU - Wahbi, L. P.

AU - Phumathon, P.

AU - Brown, A.

AU - Minter, S.

AU - Stephens, G. M.

N1 - CAN 128:12604 16-5 Fermentation and Bioindustrial Chemistry Department of Chemical Engineering, UMIST,Manchester,UK. Journal 0141-5492 written in English. 120038-36-0 (Toluene dioxygenase) Role: BAC (Biological activity or effector, except adverse), BSU (Biological study, unclassified), BIOL (Biological study) (regulated toluene cis-glycol prodn. by recombinant Escherichia coli strains constructed by PCR amplification of toluene dioxygenase genes from Pseudomonas putida); 41977-20-2P (Toluene cis-glycol) Role: BMF (Bioindustrial manufacture), BIOL (Biological study), PREP (Preparation) (regulated toluene cis-glycol prodn. by recombinant Escherichia coli strains constructed by PCR amplification of toluene dioxygenase genes from Pseudomonas putida)

PY - 1997

Y1 - 1997

N2 - The toluene dioxygenase genes from Pseudomonas pufida NCIMB 11767 were isolated by PCR amplification from recombinant plasmid, p1/1. The genes were subcloned into pUC18 and pKK223-3 and expressed under the lac and tac promoters, respectively. In both cases, toluene cis-glycol was produced, with higher levels of product formation when the genes were expressed from the tac promoter.

AB - The toluene dioxygenase genes from Pseudomonas pufida NCIMB 11767 were isolated by PCR amplification from recombinant plasmid, p1/1. The genes were subcloned into pUC18 and pKK223-3 and expressed under the lac and tac promoters, respectively. In both cases, toluene cis-glycol was produced, with higher levels of product formation when the genes were expressed from the tac promoter.

KW - Escherichia coli

KW - Molecular cloning

KW - Pseudomonas putida (regulated toluene cis-glycol prodn. by recombinant Escherichia coli strains constructed by PCR amplification of toluene dioxygenase genes from Pseudomonas putida)

KW - Gene Role: BPR (Biological process), BSU (Biological study, unclassified), BIOL (Biological study), PROC (Process) (regulated toluene cis-glycol prodn. by recombinant Escherichia coli strains constructed by PCR amplification of toluene dioxygenase genes

KW - toluene glycol prodn recombinant Escherichia dioxygenase

KW - Pseudomonas toluene dioxygenase gene cloning Escherichia

U2 - 10.1023/A:1018492630150

DO - 10.1023/A:1018492630150

M3 - Article

SN - 0141-5492

VL - 19

SP - 961

EP - 965

JO - Biotechnology Letters

JF - Biotechnology Letters

IS - 10

ER -

Regulated toluene cis-glycol production by recombinant Escherichia coli strains constructed by PCR amplification of the toluene dioxygenase genes from Pseudomonas putida

Abstract

Keywords

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