Regulation of axon growth by the JIP1-AKT axis

Federico Dajas-Bailador, Ioannis Bantounas, Emma V. Jones, Alan J. Whitmarsh

    Research output: Contribution to journalArticlepeer-review

    Abstract

    The polarisation of developing neurons to form axons and dendrites is required for the establishment of neuronal connections leading to proper brain function. The protein kinase AKT and the MAP kinase scaffold protein JNK-interacting protein-1 (JIP1) are important regulators of axon formation. Here we report that JIP1 and AKT colocalise in axonal growth cones of cortical neurons and collaborate to promote axon growth. The loss of AKT protein from the growth cone results in the degradation of JIP1 by the proteasome, and the loss of JIP1 promotes a similar fate for AKT. Reduced protein levels of both JIP1 and AKT in the growth cone can be induced by glutamate and this coincides with reduced axon growth, which can be rescued by a stabilized mutant of JIP1 that rescues AKT protein levels. Taken together, our data reveal a collaborative relationship between JIP1 and AKT that is required for axon growth and can be regulated by changes in neuronal activity. © 2014. Published by The Company of Biologists Ltd.
    Original languageEnglish
    Pages (from-to)230-239
    Number of pages9
    JournalJournal of Cell Science
    Volume127
    Issue number1
    Early online date6 Nov 2013
    DOIs
    Publication statusPublished - 1 Jan 2014

    Keywords

    • Akt
    • Axon
    • Jip1
    • Proteasome

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