Regulation of expression of the region 3 promoter of the Escherichia coli K5 capsule gene cluster involves H-NS, SlyA, and a large 5′ untranslated region7

Peng Xue, David Corbett, Marie Goldrick, Clare Naylor, Ian S. Roberts

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Escherichia coli group 2 capsule gene clusters are temperature regulated, being expressed at 37°C but not at 20°C. Expression is regulated at the level of transcription by two convergent promoters, PR1 and PR3. In this paper, we show that regulation of transcription from PR3 involves a number of novel features including H-NS, SlyA, and a large 741-bp 5′ untranslated region (UTR). H-NS represses transcription from PR3 at 20°C and binds both 5′ and 3′ of the transcription start site. The 3′ downstream regulatory element (DRE) was essential for temperature-dependent H-NS repression. At 37°C, SlyA activates transcription independent of H-NS but maximal transcription requires H-NS. The UTR is present between the transcription start site and the first gene in the operon, kpsM. We demonstrate that the UTR, as well as containing the H-NS DRE, functions to moderate the extent of transcription that reaches kpsM and allows the binding of antitermination factor RfaH. Copyright © 2009, American Society for Microbiology. All Rights Reserved.
    Original languageEnglish
    Pages (from-to)1838-1846
    Number of pages8
    JournalJournal of Bacteriology
    Volume191
    Issue number6
    DOIs
    Publication statusPublished - Mar 2009

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