Regulation of TGF beta Signalling by TRPV4 in Chondrocytes

Steven Woods (Corresponding), Paul Humphreys, Nicola Bates, Sophie Richardson, Shweta Yogesh Kuba, Immogen Brooks, Stuart Cain, Susan Kimber (Corresponding)

Research output: Contribution to journalArticlepeer-review


The growth factor TGFβ and the mechanosensitive calcium-permeable cation channel TRPV4 are both important for the development and maintenance of many tissues. Although TRPV4 and TGFβ both affect core cellular functions, how their signals are integrated is unknown. Here we show that pharmacological activation of TRPV4 significantly increased the canonical response to TGFβ stimulation in chondrocytes. Critically, this increase was only observed when TRPV4 was activated after, but not before TGFβ stimulation. The increase was prevented by pharmacological TRPV4 inhibition or knockdown and is calcium/CamKII dependent. RNA-seq analysis after TRPV4 activation showed enrichment for the TGFβ signalling pathway and identified JUN and SP1 as key transcription factors involved in this response. TRPV4 modulation of TGFβ signalling represents an important pathway linking mechanical signalling to tissue development and homeostasis.

Original languageEnglish
Article number726
Issue number4
Publication statusPublished - 24 Mar 2021


  • TGFβ signalling
  • TRPV4
  • chondrocytes


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