Projects per year
Abstract
The classical picture of enzyme catalysis relies on controlling the entropic and enthalpic contributions by manipulating reaction barriers and co-locating reactants and cofactors to facilitate the reaction chemistry. Catalysis is linked inextricably to the geometry of the enzyme-substrate complex and the chemical/physical properties of the active site, and probably to dynamical contributions that guide reactants along the desired reaction coordinate. Coenzyme B12-dependent enzymes have remarkable catalytic power and unique properties that enable detailed analysis of the reaction chemistry and associated dynamics. Here we discuss recent developments that are beginning to provide atomistic insight into how coenzyme B12- dependent enzymes steer reactants along the reaction coordinate. Such insight will ultimately generate 'movies' of the catalytic process across all relevant time scales. In the longer term, this will enable more predictive engineering of this class of enzyme to achieve new and desirable chemical outcomes. Coenzyme B12-dependent enzymes have remarkable catalytic power and unique properties that enable detailed analysis of the reaction chemistry and associated protein dynamics. Here we discuss recent developments that are beginning to provide atomistic insight of how coenzyme B12-dependent enzymes steer reactants along the reaction coordinate. Such insight will ultimately generate 'movies' of the catalytic process across all relevant timescales. © 2013 FEBS.
Original language | English |
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Pages (from-to) | 2997-3008 |
Number of pages | 11 |
Journal | FEBS Journal |
Volume | 280 |
Issue number | 13 |
DOIs | |
Publication status | Published - Jul 2013 |
Keywords
- 5'-deoxyadenosylcobalamin
- B 12
- catalysis
- dynamics
- electrostatics
- enzyme
- homolysis
- MFE
- photolysis
- radicals
Research Beacons, Institutes and Platforms
- Photon Science Institute
- Manchester Institute of Biotechnology
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Dive into the research topics of 'Relating localized protein motions to the reaction coordinate in coenzyme B12-dependent enzymes'. Together they form a unique fingerprint.Projects
- 1 Finished
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Linking experiment to theory: Quantum entanglement during enzyme catalysis - Dr S Hay fellowship
Hay, S. (PI)
1/09/10 → 31/08/15
Project: Research