Relative quantification in proteomics: new approaches for biochemistry

Richard D. Unwin, Caroline A. Evans, Anthony D. Whetton, Caroline Hargreaves

    Research output: Contribution to journalArticlepeer-review


    Recent developments in mass spectrometry and protein arrays provide opportunities to derive systematically proteomic information from small samples of cellular material. Relative quantification among samples can be achieved with either gel-based or gel-free approaches. Furthermore, the adaptation of specific techniques facilitates absolute quantification. Here, relative quantification in two-dimensional gel electrophoresis is contrasted with that in non-gel-based approaches such as isobaric tagging of peptides, pre-labelling of living cells with isotopomeric forms of essential amino acids and protein array platforms. In addition, novel flow-cytometry-based approaches are considered. These technologies can all be used to determine accurately the levels of proteins or biomarkers in a wide range of samples. © 2006 Elsevier Ltd. All rights reserved.
    Original languageEnglish
    Pages (from-to)473-484
    Number of pages11
    JournalTrends in Biochemical Sciences
    Issue number8
    Publication statusPublished - Aug 2006


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