Reproducibility of the NEPTUNE descriptor-based scoring system on whole-slide images and histologic and ultrastructural digital images.

L Barisoni, Jonathan Troost, C Nast, S Bagnasco, C Avila-Casado, J Hodgin, M Palmer, Avi Rosenberg, A Gasim, C Liensziewski, L Merlino, HP Chien, Anthony Chang, SM Meehan, J Gaut, P Song, Lawrence Holzman, D Gibson, M Kretzler, BW GillespieStephen M. Hewitt

Research output: Contribution to journalArticlepeer-review


The multicenter Nephrotic Syndrome Study Network (NEPTUNE) digital pathology scoring system employs a novel and comprehensive methodology to document pathologic features from whole-slide images, immunofluorescence and ultrastructural digital images. To estimate inter- and intra-reader concordance of this descriptor-based approach, data from 12 pathologists (eight NEPTUNE and four non-NEPTUNE) with experience from training to 30 years were collected. A descriptor reference manual was generated and a webinar-based protocol for consensus/cross-training implemented. Intra-reader concordance for 51 glomerular descriptors was evaluated on jpeg images by seven NEPTUNE pathologists scoring 131 glomeruli three times (Tests I, II, and III), each test following a consensus webinar review. Inter-reader concordance of glomerular descriptors was evaluated in 315 glomeruli by all pathologists; interstitial fibrosis and tubular atrophy (244 cases, whole-slide images) and four ultrastructural podocyte descriptors (178 cases, jpeg images) were evaluated once by six and five pathologists, respectively. Cohen's kappa for inter-reader concordance for 48/51 glomerular descriptors with sufficient observations was moderate (0.40
Original languageUndefined
Pages (from-to)671-684
Number of pages14
JournalModern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc
Publication statusPublished - 22 Apr 2016

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