Review of structural design guiding the development of lipid nanoparticles for nucleic acid delivery

Marité Cárdenas, Richard A. Campbell, Marianna Yanez arteta, M. Jayne Lawrence, Federica Sebastiani

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Abstract

Lipid nanoparticles (LNPs) are the most versatile and successful gene delivery systems, notably highlighted by their use in vaccines against COVID-19. LNPs have a well-defined core–shell structure, each region with its own distinctive compositions, suited for a wide range of in vivo delivery applications. Here, we discuss how a detailed knowledge of LNP structure can guide LNP formulation to improve the efficiency of delivery of their nucleic acid payload. Perspectives are detailed on how LNP structural design can guide more efficient nucleic acid transfection. Views on key physical characterization techniques needed for such developments are outlined including opinions on biophysical approaches both correlating structure with functionality in biological fluids and improving their ability to escape the endosome and deliver they payload.
Original languageEnglish
Article number101705
JournalCurrent Opinion in Colloid and Interface Science
Volume66
Early online date22 May 2023
DOIs
Publication statusPublished - 1 Aug 2023

Keywords

  • Lipid nanoparticles
  • Nucleic acid delicery
  • Structure-function
  • Cationic ionizable lipids

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