Abstract
Lipid nanoparticles (LNPs) are the most versatile and successful gene delivery systems, notably highlighted by their use in vaccines against COVID-19. LNPs have a well-defined core–shell structure, each region with its own distinctive compositions, suited for a wide range of in vivo delivery applications. Here, we discuss how a detailed knowledge of LNP structure can guide LNP formulation to improve the efficiency of delivery of their nucleic acid payload. Perspectives are detailed on how LNP structural design can guide more efficient nucleic acid transfection. Views on key physical characterization techniques needed for such developments are outlined including opinions on biophysical approaches both correlating structure with functionality in biological fluids and improving their ability to escape the endosome and deliver they payload.
Original language | English |
---|---|
Article number | 101705 |
Journal | Current Opinion in Colloid and Interface Science |
Volume | 66 |
Early online date | 22 May 2023 |
DOIs | |
Publication status | Published - 1 Aug 2023 |
Keywords
- Lipid nanoparticles
- Nucleic acid delicery
- Structure-function
- Cationic ionizable lipids