Rolled-up functionalized nanomembranes as three-dimensional cavities for single cell studies

Xi Wang; Christine Schmidt; Samuel Sanchez; David H. Gracias; Rafael E.  Carazo-Salas; Stephen P  Jackson; Oliver G. Schmidt

doi:10.1021/nl4042565

Rolled-up functionalized nanomembranes as three-dimensional cavities for single cell studies

Xi Wang, Christine Schmidt, Samuel Sanchez, David H. Gracias, Rafael E. Carazo-Salas, Stephen P Jackson , Oliver G. Schmidt

Division of Cancer Sciences (L5)

Research output: Contribution to journal › Article › peer-review

Abstract

We use micropatterning and strain engineering to encapsulate single living mammalian cells into transparent tubular architectures consisting of three-dimensional (3D) rolled-up nanomembranes. By using optical microscopy, we
demonstrate that these structures are suitable for the scrutiny of cellular dynamics within confined 3D-microenvironments. We show that spatial confinement of mitotic mammalian cells inside tubular architectures can perturb metaphase plate
formation, delay mitotic progression, and cause chromosomal instability in both a transformed and nontransformed human cell line. These findings could provide important clues into how spatial constraints dictate cellular behavior and function.

Original language	English
Pages (from-to)	4197–4204
Number of pages	8
Journal	Nano Letters
Volume	14
Early online date	5 Mar 2014
DOIs	https://doi.org/10.1021/nl4042565
Publication status	Published - 13 Aug 2014

Keywords

Nanomembranes, rolled-up nanotechnology, mitosis, 3D cell culture scaffold, spatial confinement, chromosome segregation errors

Research Beacons, Institutes and Platforms

Manchester Cancer Research Centre

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10.1021/nl4042565

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title = "Rolled-up functionalized nanomembranes as three-dimensional cavities for single cell studies",

abstract = "We use micropatterning and strain engineering to encapsulate single living mammalian cells into transparent tubular architectures consisting of three-dimensional (3D) rolled-up nanomembranes. By using optical microscopy, wedemonstrate that these structures are suitable for the scrutiny of cellular dynamics within confined 3D-microenvironments. We show that spatial confinement of mitotic mammalian cells inside tubular architectures can perturb metaphase plateformation, delay mitotic progression, and cause chromosomal instability in both a transformed and nontransformed human cell line. These findings could provide important clues into how spatial constraints dictate cellular behavior and function.",

keywords = "Nanomembranes, rolled-up nanotechnology, mitosis, 3D cell culture scaffold, spatial confinement, chromosome segregation errors",

author = "Xi Wang and Christine Schmidt and Samuel Sanchez and Gracias, {David H.} and Carazo-Salas, {Rafael E.} and Jackson, {Stephen P} and Schmidt, {Oliver G.}",

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doi = "10.1021/nl4042565",

language = "English",

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journal = "Nano Letters",

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T1 - Rolled-up functionalized nanomembranes as three-dimensional cavities for single cell studies

AU - Wang, Xi

AU - Schmidt, Christine

AU - Sanchez, Samuel

AU - Gracias, David H.

AU - Carazo-Salas, Rafael E.

AU - Jackson , Stephen P

AU - Schmidt, Oliver G.

PY - 2014/8/13

Y1 - 2014/8/13

N2 - We use micropatterning and strain engineering to encapsulate single living mammalian cells into transparent tubular architectures consisting of three-dimensional (3D) rolled-up nanomembranes. By using optical microscopy, wedemonstrate that these structures are suitable for the scrutiny of cellular dynamics within confined 3D-microenvironments. We show that spatial confinement of mitotic mammalian cells inside tubular architectures can perturb metaphase plateformation, delay mitotic progression, and cause chromosomal instability in both a transformed and nontransformed human cell line. These findings could provide important clues into how spatial constraints dictate cellular behavior and function.

AB - We use micropatterning and strain engineering to encapsulate single living mammalian cells into transparent tubular architectures consisting of three-dimensional (3D) rolled-up nanomembranes. By using optical microscopy, wedemonstrate that these structures are suitable for the scrutiny of cellular dynamics within confined 3D-microenvironments. We show that spatial confinement of mitotic mammalian cells inside tubular architectures can perturb metaphase plateformation, delay mitotic progression, and cause chromosomal instability in both a transformed and nontransformed human cell line. These findings could provide important clues into how spatial constraints dictate cellular behavior and function.

KW - Nanomembranes, rolled-up nanotechnology, mitosis, 3D cell culture scaffold, spatial confinement, chromosome segregation errors

U2 - 10.1021/nl4042565

DO - 10.1021/nl4042565

M3 - Article

SN - 1530-6984

VL - 14

SP - 4197

EP - 4204

JO - Nano Letters

JF - Nano Letters

ER -

Rolled-up functionalized nanomembranes as three-dimensional cavities for single cell studies

Abstract

Keywords

Research Beacons, Institutes and Platforms

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