@inbook{91bb8cf165a34db0a39a08f5411a6713,
title = "RT-PCR for the detection of translocations in bone and soft tissue tumours in formalin-fixed paraffin-embedded tissues",
abstract = "This chapter outlines the methodology for the detection of mesenchymal-tumour-specific translocations in formalin-fixed paraffin-embedded tissue (FFPET) using the reverse transcriptase-polymerase chain reaction (RT-PCR). It includes the design of appropriate primer pairs and the necessary pretreatment of the FFPET sections to give the maximum yield of analyzable RNA, in terms of both quantity and quality. {\textcopyright} 2011 Humana Press.",
keywords = "Acid decalcification, Formalin-fixed paraffin-embedded tissue (FFPET), RT-PCR, Sarcoma-specific transl ocations",
author = "Ann Williams and Mangham, {D. Chas}",
year = "2011",
doi = "10.1007/978-1-60761-947-5_17",
language = "English",
volume = "688",
series = "Methods in Molecular Biology",
pages = "257--268",
booktitle = "Methods in Molecular Biology|Methods Mol. Biol.",
}
