RT-PCR for the detection of translocations in bone and soft tissue tumours in formalin-fixed paraffin-embedded tissues

Ann Williams, D. Chas Mangham

    Research output: Chapter in Book/Report/Conference proceedingChapter

    Abstract

    This chapter outlines the methodology for the detection of mesenchymal-tumour-specific translocations in formalin-fixed paraffin-embedded tissue (FFPET) using the reverse transcriptase-polymerase chain reaction (RT-PCR). It includes the design of appropriate primer pairs and the necessary pretreatment of the FFPET sections to give the maximum yield of analyzable RNA, in terms of both quantity and quality. © 2011 Humana Press.
    Original languageEnglish
    Title of host publicationMethods in Molecular Biology|Methods Mol. Biol.
    Pages257-268
    Number of pages11
    Volume688
    DOIs
    Publication statusPublished - 2011

    Publication series

    NameMethods in Molecular Biology

    Keywords

    • Acid decalcification
    • Formalin-fixed paraffin-embedded tissue (FFPET)
    • RT-PCR
    • Sarcoma-specific transl ocations

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