S phase progression in human cells is dictated by the genetic continuity of DNA foci

Pedro Olivares Chauvet, Apolinar Maya-Mendoza, Pedro Olivares-Chauvet, Alex Shaw, Dean A. Jackson

    Research output: Contribution to journalArticlepeer-review

    Abstract

    DNA synthesis must be performed with extreme precision to maintain genomic integrity. In mammalian cells, different genomic regions are replicated at defined times, perhaps to preserve epigenetic information and cell differentiation status. However, the molecular principles that define this S phase program are unknown. By analyzing replication foci within discrete chromosome territories during interphase, we show that foci which are active during consecutive intervals of S phase are maintained as spatially adjacent neighbors throughout the cell cycle. Using extended DNA fibers, we demonstrate that this spatial continuity of replication foci correlates with the genetic continuity of adjacent replicon clusters along chromosomes. Finally, we used bioinformatic tools to compare the structure of DNA foci with DNA domains that are seen to replicate during discrete time intervals of S phase using genome-wide strategies. Data presented show that a major mechanism of S phase progression involves the sequential synthesis of regions of the genome because of their genetic continuity along the chromosomal fiber. © 2010 Maya-Mendoza et al.
    Original languageEnglish
    JournalPL o S Genetics
    Volume6
    Issue number4
    DOIs
    Publication statusPublished - Apr 2010

    Fingerprint

    Dive into the research topics of 'S phase progression in human cells is dictated by the genetic continuity of DNA foci'. Together they form a unique fingerprint.

    Cite this