Abstract
Background: Quiescent CD4 + T lymphocytes are highly refractory to HIV-1 infection due to a block at reverse transcription.Results: Examination of SAMHD1 expression in peripheral blood lymphocytes shows that SAMHD1 is expressed in both CD4+ and CD8+ T cells at levels comparable to those found in myeloid cells. Treatment of CD4+ T cells with Virus-Like Particles (VLP) containing Vpx results in the loss of SAMHD1 expression that correlates with an increased permissiveness to HIV-1 infection and accumulation of reverse transcribed viral DNA without promoting transcription from the viral LTR. Importantly, CD4 + T-cells from patients with Aicardi-Goutières Syndrome harboring mutation in the SAMHD1 gene display an increased susceptibility to HIV-1 infection that is not further enhanced by VLP-Vpx-treatment.Conclusion: Here, we identified SAMHD1 as the restriction factor preventing efficient viral DNA synthesis in non-cycling resting CD4 + T-cells. These results highlight the crucial role of SAMHD1 in mediating restriction of HIV-1 infection in quiescent CD4 + T-cells and could impact our understanding of HIV-1 mediated CD4 + T-cell depletion and establishment of the viral reservoir, two of the HIV/AIDS hallmarks. © 2012 Descours et al.; licensee BioMed Central Ltd.
Original language | English |
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Article number | 87 |
Journal | Retrovirology |
Volume | 9 |
DOIs | |
Publication status | Published - 23 Oct 2012 |
Keywords
- HIV-1
- Quiescent CD4 + T-cell
- Restriction
- Reverse transcription
- SAMHD1