Sampling Microbiomes Associated with Different Plant Compartments

Henry W G Birt; Anthony B Pattison; Paul G Dennis

doi:10.1007/978-1-0716-1040-4_2

Sampling Microbiomes Associated with Different Plant Compartments

Henry W G Birt, Anthony B Pattison, Paul G Dennis

Earth and Environmental Sciences - Academic & Research

Research output: Contribution to journal › Article › peer-review

Abstract

The microbiome is known to influence plant fitness and differs significantly between plant compartments. To characterize the communities associated with different plant compartments, it is necessary to separate plant tissues in a manner that is suitable for microbiome analysis. Here, we describe a standardized protocol for sampling the microbiomes associated with bulk soil, the apical and basal ectorhizosphere, the apical and ectorhizosphere, the rhizome, pseudostem, and leaves of Musa spp. The approach can easily be modified for work with other plants.

Original language	English
Pages (from-to)	23-29
Number of pages	7
Journal	Methods in molecular biology (Clifton, N.J.)
Volume	2232
Early online date	8 Nov 2020
DOIs	https://doi.org/10.1007/978-1-0716-1040-4_2
Publication status	Published - 2021

Keywords

Microbiota/genetics
Molecular Biology/methods
Musa/genetics
Plant Leaves/genetics
Plant Roots/genetics
Rhizome/genetics

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10.1007/978-1-0716-1040-4_2

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title = "Sampling Microbiomes Associated with Different Plant Compartments",

abstract = "The microbiome is known to influence plant fitness and differs significantly between plant compartments. To characterize the communities associated with different plant compartments, it is necessary to separate plant tissues in a manner that is suitable for microbiome analysis. Here, we describe a standardized protocol for sampling the microbiomes associated with bulk soil, the apical and basal ectorhizosphere, the apical and ectorhizosphere, the rhizome, pseudostem, and leaves of Musa spp. The approach can easily be modified for work with other plants.",

keywords = "Microbiota/genetics, Molecular Biology/methods, Musa/genetics, Plant Leaves/genetics, Plant Roots/genetics, Rhizome/genetics",

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year = "2021",

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AU - Pattison, Anthony B

AU - Dennis, Paul G

PY - 2021

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AB - The microbiome is known to influence plant fitness and differs significantly between plant compartments. To characterize the communities associated with different plant compartments, it is necessary to separate plant tissues in a manner that is suitable for microbiome analysis. Here, we describe a standardized protocol for sampling the microbiomes associated with bulk soil, the apical and basal ectorhizosphere, the apical and ectorhizosphere, the rhizome, pseudostem, and leaves of Musa spp. The approach can easily be modified for work with other plants.

KW - Microbiota/genetics

KW - Molecular Biology/methods

KW - Musa/genetics

KW - Plant Leaves/genetics

KW - Plant Roots/genetics

KW - Rhizome/genetics

UR - https://www.scopus.com/pages/publications/85095917612

U2 - 10.1007/978-1-0716-1040-4_2

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SN - 1064-3745

VL - 2232

SP - 23

EP - 29

JO - Methods in molecular biology (Clifton, N.J.)

JF - Methods in molecular biology (Clifton, N.J.)

ER -

Sampling Microbiomes Associated with Different Plant Compartments

Abstract

Keywords

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