Segmental sodium reabsorption by the renal tubule in prenatally programmed hypertension in the rat

Saleh H. Alwasel; Nick Ashton

doi:10.1007/s00467-011-1976-9

Segmental sodium reabsorption by the renal tubule in prenatally programmed hypertension in the rat

Saleh H. Alwasel, Nick Ashton

Research output: Contribution to journal › Article › peer-review

Abstract

Hypertension and renal dysfunction can be programmed in the rat by prenatal exposure to a lowprotein (LP) diet. Expression of the renal thick ascending limb (TAL) sodium transporter NKCC2 is up-regulated, which has been predicted to result in greater sodium reabsorption. However, we have shown that LP rats excrete more not less sodium. The aim of this study was to determine whether the increased abundance of sodium: Potassium:chloride (Na+:K+:2Cl-) co-transporter (NKCC2) leads to enhanced sodium uptake by the TAL. Pregnant Wistar rats were fed a control (18%) or LP (9%) diet. Amiloride (AM), bendroflumethiazide (BF), and furosemide (FUR) were administered acutely to male offspring at 4 weeks of age. Fractional excretion of sodium (FENa) was significantly greater in vehicle-infused LP rats (3.0±0.3%) compared with controls (1.7±0.5, P

Original language	English
Pages (from-to)	285-293
Number of pages	8
Journal	Pediatric Nephrology
Volume	27
Issue number	2
DOIs	https://doi.org/10.1007/s00467-011-1976-9
Publication status	Published - Feb 2012

Keywords

Bendroflumethiazide
Furosemide
Kidney
NKCC2
Pressure natriuresis
Programmed hypertension
Sodium

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10.1007/s00467-011-1976-9

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title = "Segmental sodium reabsorption by the renal tubule in prenatally programmed hypertension in the rat",

abstract = "Hypertension and renal dysfunction can be programmed in the rat by prenatal exposure to a lowprotein (LP) diet. Expression of the renal thick ascending limb (TAL) sodium transporter NKCC2 is up-regulated, which has been predicted to result in greater sodium reabsorption. However, we have shown that LP rats excrete more not less sodium. The aim of this study was to determine whether the increased abundance of sodium: Potassium:chloride (Na+:K+:2Cl-) co-transporter (NKCC2) leads to enhanced sodium uptake by the TAL. Pregnant Wistar rats were fed a control (18%) or LP (9%) diet. Amiloride (AM), bendroflumethiazide (BF), and furosemide (FUR) were administered acutely to male offspring at 4 weeks of age. Fractional excretion of sodium (FENa) was significantly greater in vehicle-infused LP rats (3.0±0.3%) compared with controls (1.7±0.5, P",

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AU - Alwasel, Saleh H.

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KW - Bendroflumethiazide

KW - Furosemide

KW - Kidney

KW - NKCC2

KW - Pressure natriuresis

KW - Programmed hypertension

KW - Sodium

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DO - 10.1007/s00467-011-1976-9

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EP - 293

JO - Pediatric Nephrology

JF - Pediatric Nephrology

IS - 2

ER -

Segmental sodium reabsorption by the renal tubule in prenatally programmed hypertension in the rat

Abstract

Keywords

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