Segregation of genomes in polyploid tumour cells following mitotic catastrophe

Jekaterina Erenpreisa, M. Kalejs, F. Ianzini, E. A. Kosmacek, M. A. MacKey, D. Emzinsh, M. S. Cragg, A. Ivanov, T. M. Illidge

    Research output: Contribution to journalArticlepeer-review


    Following irradiation p53-function-deficient tumour cells undergo mitotic catastrophe and form endopolyploid cells. A small proportion of these segregates nuclei, and give rise to viable descendants. Here we studied this process in five tumour cell lines. After mitotic failure, tumour cells enter the endocycle and form mono-nucleated or multi-nucleated giant cells (MOGC and MNGC). MNGC arise from arrested anaphases, MOGC, from arrested metaphases. In both cases the individual genomes establish a radial pattern by links to a single microtubule organizing centre. Segregation of genomes is also ordered. MNGC present features of mitosis being resumed from late anaphase. In MOGC the sub-nuclei retain arrangement of stacked metaphase plates and are separated by folds of the nuclear envelope. Mitosis then resumes in sub-nuclei directly from metaphase. The data presented indicate that endopolyploid tumour cells preserve the integrity of individual genomes and can potentially re-initiate mitosis from the point at which it was interrupted. © 2005 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved.
    Original languageEnglish
    Pages (from-to)1005-1011
    Number of pages6
    JournalCell Biology International
    Issue number12
    Publication statusPublished - Dec 2005


    • De-polyploidisation
    • Link to microtubule-organising centre
    • Mitotic catastrophe
    • Radial arrangement of genomes
    • Resuming of mitosis


    Dive into the research topics of 'Segregation of genomes in polyploid tumour cells following mitotic catastrophe'. Together they form a unique fingerprint.

    Cite this